Drug release performance

1. To conduct the drug release performance of LGMC, semicircular LGMC was sticking to a glass slide at first. As the following diagram shows, a 0.3 mm capillary tube was placed on a glass slide, then the optimized PVDF porous membrane fabricated with 15.9% mass ratio was skinning on the capillary tube. Two cover glasses were used to pinning the membrane, and after affixed with epoxy resin glue, capillary tube was drawn out. Following with the infusion of gating liquid, semicircular membrane-based LGMC was obtained. In this way, the semicircular LGMC has a radius of about 0.3 mm, and the material was PVDF porous membrane fabricated with 15.9% wt/wt electrospinning solution.

2. To detect the drug release performance, confocal laser scanning microscope (TCS SP5, Leica) was used, with which xzy mode can be realized, and fluorescent dyesdissolve in silicone oil 500 were used as indicators. While semicircular membrane-based LGMC was in a focused place, with slowly flowed DI water as transport fluid, fluorescent dyes (1  ml) was dropped on the LGMC near the laser focused position with a microsyringe, and at the same time, confocal images were recorded. Both oil-soluble (fluorescent yellow 131SC) and water-soluble (rhodamine B) molecules were used, respectively.