Bio-101 - Improve Research Reproducibility

Experimental models

Authors: Hongxue Shi and Ira Tabas
Date: Nov 28, 2022，Views：858

Original protocol used in research article：

CD47-SIRPα axis blockade in NASH promotes necroptotic hepatocyte clearance by liver macrophages and decreases hepatic fibrosis
Science Translational Medicine, Nov 23, 2022; DOI: 10.1126/scitranslmed.abp8309

Whole mount in situ hybridization (WISH)

Author: Feng Liu
Date: Nov 28, 2022，Views：867

Original protocol used in research article：

The chromatin-remodeling enzyme Smarca5 regulates erythrocyte aggregation via Keap1-Nrf2 signaling
eLife, Oct 26, 2021; DOI: 10.7554/eLife.72557

2.4. CDC and ADCC assays

Author: Kohtaro Minmi
Date: Nov 27, 2022，Views：887

Original protocol used in research article：

Nonclinical safety evaluation of pabinafusp alfa, an anti-human transferrin receptor antibody and iduronate-2-sulfatase fusion protein, for the treatment of neuronopathic mucopolysaccharidosis type II
Molecular Genetics and Metabolism Reports, Apr 18, 2021; DOI: 10.1016/j.ymgmr.2021.100758

Zebrafish strains

Authors: Yanyan Ding and Feng Liu
Date: Nov 25, 2022，Views：803

Original protocol used in research article：

The chromatin-remodeling enzyme Smarca5 regulates erythrocyte aggregation via Keap1-Nrf2 signaling
eLife, Oct 26, 2021; DOI: 10.7554/eLife.72557

Preparation of exosome and exosomeRNP

Authors: Xiangrui Liu, Yuan Ping, Tianhua Zhou and Tao Wan
Date: Nov 25, 2022，Views：945

Original protocol used in research article：

Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases
Science Advances, Sep 14, 2022; DOI: 10.1126/sciadv.abp9435

Drug release performance

Author: Xu Hou
Date: Nov 25, 2022，Views：746

Original protocol used in research article：

Bioinspired liquid gating membrane-based catheter with anticoagulation and positionally drug release properties
Science Advances, Sep 3, 2020; DOI: 10.1126/sciadv.abb4700

Plasmid constructs and plant transformation

Author: Zuhua He
Date: Nov 25, 2022，Views：775

Original protocol used in research article：

An MKP-MAPK protein phosphorylation cascade controls vascular immunity in plants
Science Advances, Mar 9, 2022; DOI: 10.1126/sciadv.abg8723

2.2. Determination of Total Flavonoids Content

Authors: Xiaoqing Ma, Qiang Ding, Xilin Hou and Xiong You
Date: Nov 24, 2022，Views：697

Original protocol used in research article：

Analysis of Flavonoid Metabolites in Watercress (Nasturtium officinale R. Br.) and the Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis cv. Aijiaohuang) Using UHPLC-ESI-MS/MS
Molecules, Sep 26, 2021; DOI: 10.3390/molecules26195825

Axenic flies

Authors: Katarina Akhmetova and Igor Chesnokov
Date: Nov 22, 2022，Views：723

Original protocol used in research article：

Drosophila STING protein has a role in lipid metabolism
eLife, Sep 1, 2021; DOI: 10.7554/eLife.67358

Generation of INKBRITE mouse model

Author: Tien Peng
Date: Nov 21, 2022，Views：868

Original protocol used in research article：

Sentinel p16INK4a+ cells in the basement membrane form a reparative niche in the lung
Science, Oct 13, 2022; DOI: 10.1126/science.abf3326

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