Protocols shared by authors via RaP service
Journal
DNA extraction, 16S rRNA sequencing and data analysis
Authors: Jiliang Hu and Jeff Gore
Date: May 26, 2023，Views：46
Original protocol used in research article：
Emergent phases of ecological diversity and dynamics mapped in microcosms
Science, Oct 6, 2022; DOI: 10.1126/science.abm7841
Lipid Extraction From Cultured Adipocytes
Authors: John Se Kit Yuen Jr and David L Kaplan
Date: May 25, 2023，Views：978
Original protocol used in research article：
Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
eLife, Apr 4, 2023; DOI: 10.7554/eLife.82120
Plasmodium cynomolgi sporozoite production and sporozoite isolation
Authors: Devendra Kumar Gupta and Thierry Tidiane Diagana
Date: May 24, 2023，Views：959
Original protocol used in research article：
The Plasmodium liver-specific protein 2 (LISP2) is an early marker of liver stage development
eLife, May 16, 2019; DOI: 10.7554/eLife.43362
3.5.2. TBARS Assay
Authors: Fatma M. Elessawy and Randy W. Purves
Date: May 24, 2023，Views：929
Original protocol used in research article：
An Untargeted Metabolomics Approach for Correlating Pulse Crop Seed Coat Polyphenol Profiles with Antioxidant Capacity and Iron Chelation Ability
Molecules, Jun 23, 2021; DOI: 10.3390/molecules26133833
EdU labeling assay
Authors: Akansha M. Shah, Eric N. Olson and Ning Liu
Date: May 23, 2023，Views：830
Original protocol used in research article：
TWIST2-mediated chromatin remodeling promotes fusion-negative rhabdomyosarcoma
Science Advances, Apr 28, 2023; DOI: 10.1126/sciadv.ade8184
Protein purification, MST, and EMSAs
Authors: Shengze Yao and Yi Li
Date: May 23, 2023，Views：710
Original protocol used in research article：
The key micronutrient copper orchestrates broad-spectrum virus resistance in rice
Science Advances, Jul 1, 2022; DOI: 10.1126/sciadv.abm0660
Purification of DNA Origami Nanostructures
Authors: Pierre Stömmer and Hendrik Dietz
Date: May 23, 2023，Views：704
Original protocol used in research article：
Cryo-Electron Microscopy and Mass Analysis of Oligolysine-Coated DNA Nanostructures
ACS Nano, Mar 16, 2021; DOI: 10.1021/acsnano.0c10137
Data acquisition of resting-state fMRI and optogenetic fMRI experiments
Authors: Jiaming Liu, Ed X. Wu, Yanqiu Feng and Xiong Cao
Date: May 23, 2023，Views：618
Original protocol used in research article：
Astrocyte dysfunction drives abnormal resting-state functional connectivity in depression
Science Advances, Nov 16, 2022; DOI: 10.1126/sciadv.abo2098
Macrophage depletion and reconstitution
Authors: Kang Yi Su and Wei-Jia Luo
Date: May 23, 2023，Views：545
Original protocol used in research article：
HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages
eLife, ; DOI: 10.7554/eLife.76094
PLZF knockdown using siRNA
Author: Shuchi Agrawal Singh
Date: May 22, 2023，Views：508
Original protocol used in research article：
PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells
eLife, Jan 23, 2019; DOI: 10.7554/eLife.40364
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