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A high-quality database for basic life science protocols
Protocols
Research fields
Biochemistry (1361)
Biophysics (158)
Cancer Biology (423)
Cell Biology (2085)
Developmental Biology (314)
Immunology (592)
Microbiology (1142)
Molecular Biology (1113)
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Authors:
Jose Felix Moruno Manchon
and
Andrey S Tsvetkov
Date: Mar 3, 2022,Views:634
Original protocol used in research article:
Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons
eLife, Feb 11, 2020; DOI:
10.7554/eLife.52283
Cell cultures and transfection
Authors:
Jose Felix Moruno Manchon
and
Andrey S Tsvetkov
Date: Mar 3, 2022,Views:610
Original protocol used in research article:
Small-molecule G-quadruplex stabilizers reveal a novel pathway of autophagy regulation in neurons
eLife, Feb 11, 2020; DOI:
10.7554/eLife.52283
DiI tracing
Authors:
Nicolas Renier
and
Alain Chédotal
Date: Mar 3, 2022,Views:618
Original protocol used in research article:
A mutant with bilateral whisker to barrel inputs unveils somatosensory mapping rules in the cerebral cortex
eLife, Mar 28, 2017; DOI:
10.7554/eLife.23494
Selection of genetic instruments
Authors:
Wuqing Huang
,
Jun Xiao
,
Jianguang Ji
and
Liangwan Chen
Date: Mar 2, 2022,Views:699
Original protocol used in research article:
Association of lipid-lowering drugs with COVID-19 outcomes from a Mendelian randomization study
eLife, Dec 6, 2021; DOI:
10.7554/eLife.73873
Microinjection and selection and confirmation of transgenic zebrafish
Author:
Chiou-Hwa Yuh
Date: Mar 2, 2022,Views:567
Original protocol used in research article:
l-Carnitine ameliorates congenital myopathy in a tropomyosin 3 de novo mutation transgenic zebrafish
Journal of Biomedical Science, Jan 12, 2021; DOI:
10.1186/s12929-020-00707-1
Cell culture, transfection, and drug treatments
Authors:
Jason E. Lee
and
Gia K. Voeltz
Date: Mar 2, 2022,Views:613
Original protocol used in research article:
Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI:
10.1126/science.aay7108
Cell activation and immunostaining
Author:
Jesse Goyette
Date: Mar 2, 2022,Views:631
Original protocol used in research article:
Protein-PAINT: Superresolution microscopy with signaling proteins
Science Signaling, Feb 1, 2022; DOI:
10.1126/scisignal.abg9782
Conjunction of DNA and primary antibody
Authors:
Xiaohao Liu
,
Fenyong Sun
,
Yu Yang
,
Xiaoli Zhu
and
Weihong Tan
Date: Mar 2, 2022,Views:686
Original protocol used in research article:
Computer-aided design of reversible hybridization chain reaction (CAD-HCR) enables multiplexed single-cell spatial proteomics imaging
Science Advances, Jan 14, 2022; DOI:
10.1126/sciadv.abk0133
Islet dispersion
Author:
J. E. Campbell
Date: Mar 2, 2022,Views:605
Original protocol used in research article:
GIP mediates the incretin effect and glucose tolerance by dual actions on α cells and β cells
Science Advances, Mar 12, 2021; DOI:
10.1126/sciadv.abf1948
Western blotting
Authors:
Sthitapranjya Pati
and
Vidita A Vaidya
Date: Mar 1, 2022,Views:723
Original protocol used in research article:
Chronic postnatal chemogenetic activation of forebrain excitatory neurons evokes persistent changes in mood behavior
eLife, Sep 21, 2020; DOI:
10.7554/eLife.56171
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