Bio-101 - Improve Research Reproducibility

CRISPR knockout and siRNA knockdown

Authors: Zhiping Weng and Kevin Struhl
Date: Mar 22, 2022，Views：700

Original protocol used in research article：

YAP and TAZ are transcriptional co-activators of AP-1 proteins and STAT3 during breast cellular transformation
eLife, Aug 31, 2021; DOI: 10.7554/eLife.67312

Culture conditions

Authors: Milo S Johnson and Michael M Desai
Date: Mar 21, 2022，Views：706

Original protocol used in research article：

Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations
eLife, Jan 19, 2021; DOI: 10.7554/eLife.63910

Supervised classification

Authors: Diptendu Mukherjee, Eyal Itskovits and Ami Citri
Date: Mar 21, 2022，Views：612

Original protocol used in research article：

Salient experiences are represented by unique transcriptional signatures in the mouse brain
eLife, Feb 7, 2018; DOI: 10.7554/eLife.31220

Expression and preparation of proteins

Authors: Tao Xie, Tamjeed Saleh, Paolo Rossi and Charalampos G. Kalodimos
Date: Mar 21, 2022，Views：654

Original protocol used in research article：

Conformational states dynamically populated by a kinase determine its function
Science, Oct 8, 2020; DOI: 10.1126/science.abc2754

Preparation for two-photon calcium imaging in visual cortex

Authors: Carsen Stringer, Marius Pachitariu and Kenneth D. Harris
Date: Mar 18, 2022，Views：606

Original protocol used in research article：

Spontaneous behaviors drive multidimensional, brainwide activity
Science, Apr 19, 2019; DOI: 10.1126/science.aav7893

Generation of erythroid-specific TLR9-deficient mice

Authors: LK Metthew Lam, Kaitlyn Eckart and Nilam S. Mangalmurti
Date: Mar 17, 2022，Views：713

Original protocol used in research article：

DNA binding to TLR9 expressed by red blood cells promotes innate immune activation and anemia
Science Translational Medicine, Oct 20, 2021; DOI: 10.1126/scitranslmed.abj1008

Rabbit bioassay of in vivo insulin glargine activity

Authors: Parag Goyal and Ramakrishnan Melarkode
Date: Mar 17, 2022，Views：584

Original protocol used in research article：

Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine
PLoS ONE, Jun 16, 2021; DOI: 10.1371/journal.pone.0253168

αCD45 infusions

Authors: E. Lake Potter and Mario Roederer
Date: Mar 16, 2022，Views：657

Original protocol used in research article：

Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining
Science Translational Medicine, Jan 13, 2021; DOI: 10.1126/scitranslmed.abb4582

Nile red membrane staining

Author: Ronit Vogt Sionov
Date: Mar 16, 2022，Views：857

Original protocol used in research article：

Anandamide alters the membrane properties, halts the cell division and prevents drug efflux in multidrug resistant Staphylococcus aureus
Scientific Reports, Apr 22, 2021; DOI: 10.1038/s41598-021-88099-6

LRP10 CRISPR/Cas9-mediated knockout in HuTu 80 cells

Authors: Martyna Grochowska and Wim Mandemakers
Date: Mar 14, 2022，Views：648

Original protocol used in research article：

LRP10 interacts with SORL1 in the intracellular vesicle trafficking pathway in non-neuronal brain cells and localises to Lewy bodies in Parkinson’s disease and dementia with Lewy bodies
Acta Neuropathol, Apr 28, 2021; DOI: 10.1007/s00401-021-02313-3

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