Protocols shared by authors via RaP service
Journal
RT-qPCR
Authors: Colin J Dinsmore and Philippe Soriano
Date: Sep 8, 2022，Views：638
Original protocol used in research article：
Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors
eLife, Jan 19, 2022; DOI: 10.7554/eLife.75106
Detection of SARS-CoV-2 genomic RNA in nasal swabs and lung homogenates by quantitative reverse transcription polymerase chain reaction
Authors: Stephanie N. Langel and Sean N. Tucker
Date: Sep 8, 2022，Views：533
Original protocol used in research article：
Adenovirus type 5 SARS-CoV-2 vaccines delivered orally or intranasally reduced disease severity and transmission in a hamster model
Science Translational Medicine, May 5, 2022; DOI: 10.1126/scitranslmed.abn6868
Fly stocks and food
Authors: Colin D McClure and Tony D Southall
Date: Sep 8, 2022，Views：716
Original protocol used in research article：
An auxin-inducible, GAL4-compatible, gene expression system for Drosophila
eLife, ; DOI: 10.7554/eLife.67598
Yeast toxicity assays
Authors: Zhao-Qing Luo and Jiaqi Fu
Date: Sep 8, 2022，Views：536
Original protocol used in research article：
Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases
eLife, Jan 27, 2022; DOI: 10.7554/eLife.73611
Crystal violet assay
Authors: Lisanne de Vor and Suzan HM Rooijakkers
Date: Sep 8, 2022，Views：562
Original protocol used in research article：
Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm
eLife, Jan 6, 2022; DOI: 10.7554/eLife.67301
Single-cell/-nucleus processing for sc/snRNA-seq
Authors: Rebecca D Hodge and Bosiljka Tasic
Date: Sep 7, 2022，Views：601
Original protocol used in research article：
Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans
eLife, Sep 2, 2021; DOI: 10.7554/eLife.64875
Measures
Author: Andrew S. Huhn
Date: Sep 7, 2022，Views：524
Original protocol used in research article：
Suvorexant ameliorated sleep disturbance, opioid withdrawal, and craving during a buprenorphine taper
Science Translational Medicine, Jun 22, 2022; DOI: 10.1126/scitranslmed.abn8238
CytoTRACE analysis in the mammary epithelial atlas paper (https://doi.org/10.1038/s42003-021-02201-2)
Authors: Kohei Saeki and Shiuan Chen
Date: Sep 6, 2022，Views：527
Original protocol used in research article：
Mammary cell gene expression atlas links epithelial cell remodeling events to breast carcinogenesis
Communications Biology, Jun 2, 2021; DOI: 10.1038/s42003-021-02201-2
Craniofacial morphology by micro-CT
Authors: Nandini Singh and Roger H Reeves
Date: Sep 6, 2022，Views：535
Original protocol used in research article：
A non-mosaic transchromosomic mouse model of Down syndrome carrying the long arm of human chromosome 21
eLife, Jun 29, 2020; DOI: 10.7554/eLife.56223
Mouse tissue collection and assessments
Authors: Dominik Saul, Joshua N Farr and Sundeep Khosla
Date: Sep 6, 2022，Views：533
Original protocol used in research article：
Modulation of fracture healing by the transient accumulation of senescent cells
eLife, Oct 7, 2021; DOI: 10.7554/eLife.69958
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