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Preprint

pMHCII-CAR constructs

JY Jaeu Yi
GW Gregory F. Wu
CH Chyi-Song Hsieh

Last updated date: Nov 5, 2022 Views: 578 Forks: 0

original An abbreviated version of this protocol was published in Science Immunology
Antigen-specific depletion of CD4+ T cells by CAR T cells reveals distinct roles of higher- and lower-affinity TCRs during autoimmunity
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Please refer to the attached ppt file for detailed information of pMHCII CAR vector. OVA pMHCII CAR is displayed as example.

These were cloned into MigR vector.

 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Yi, J, Wu, G and Hsieh, C(2022). pMHCII-CAR constructs. Bio-protocol Preprint. bio-protocol.org/prep2031.
  2. Yi, J., Miller, A. T., Archambault, A. S., Jones, A. J., Bradstreet, T. R., Bandla, S., Hsu, Y., Edelson, B. T., Zhou, Y. W., Fremont, D. H., Egawa, T., Singh, N., Wu, G. F. and Hsieh, C.(2022). Antigen-specific depletion of CD4+ T cells by CAR T cells reveals distinct roles of higher- and lower-affinity TCRs during autoimmunity. Science Immunology 7(76). DOI: 10.1126/sciimmunol.abo0777

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