Protocols shared by authors via RaP service
Journal
Quantification of fungal growth by qPCR
Author: Shouan Liu
Date: Oct 12, 2022，Views：342
Original protocol used in research article：
Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100
eLife, Jun 15, 2015; DOI: 10.7554/eLife.07295
Single animal PCR genotyping protocol for C. elegans
Authors: Jian Chen and Tim Schedl
Date: Oct 10, 2022，Views：541
Original protocol used in research article：
A simple one-step PCR assay for SNP detection
microPublication Biology, Jun 11, 2021; DOI: 10.17912/micropub.biology.000399
Dual-Luciferase Transient Expression Assays of Arabidopsis Protoplasts
Authors: Jun Zhu and Zhong-Nan Yang
Date: Oct 9, 2022，Views：497
Original protocol used in research article：
OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice
Rice, Jan 6, 2021; DOI: 10.1186/s12284-020-00451-y
In-home radio-based gait monitoring
Author: Yingcheng Liu
Date: Oct 7, 2022，Views：392
Original protocol used in research article：
Monitoring gait at home with radio waves in Parkinson’s disease: A marker of severity, progression, and medication response
Science Translational Medicine, Sep 21, 2022; DOI: 10.1126/scitranslmed.adc9669
Hi-C data processing and A/B compartment calculation
Authors: Kei Fukuda and Yoichi Shinkai
Date: Oct 6, 2022，Views：371
Original protocol used in research article：
Regulation of mammalian 3D genome organization and histone H3K9 dimethylation by H3K9 methyltransferases
Communications Biology, May 13, 2021; DOI: 10.1038/s42003-021-02089-y
Inducing blunt force trauma in third-instar Drosophila melanogaster larvae using the High-Impact Trauma (HIT) device
Authors: Jorgo Lika, Rebeccah J Katzenberger, Barry Ganetzky and David A Wassarman
Date: Oct 6, 2022，Views：284
Original protocol used in research article：
Effects of blunt force injuries in third-instar Drosophila larvae persist through metamorphosis and reduce adult lifespan
microPublication Biology, ; DOI: 10.17912/micropub.biology.000423
Immunofluorescence staining
Authors: Shijie Liu and James F. Martin
Date: Oct 5, 2022，Views：398
Original protocol used in research article：
Gene therapy knockdown of Hippo signaling induces cardiomyocyte renewal in pigs after myocardial infarction
Science Translational Medicine, Jun 30, 2021; DOI: 10.1126/scitranslmed.abd6892
Design and preparation of crRNA
Authors: Fuchang Yu and Longxian Zhang
Date: Oct 5, 2022，Views：326
Original protocol used in research article：
CRISPR/Cas12a-based on-site diagnostics of Cryptosporidium parvum IId-subtype-family from human and cattle fecal samples
Parasites & Vectors, Apr 20, 2021; DOI: 10.1186/s13071-021-04709-2
Glutathione Peroxidase Activity Assay
Authors: Yafang Zhang and Huike Yang
Date: Oct 3, 2022，Views：378
Original protocol used in research article：
Crocin Improves Endothelial Mitochondrial Dysfunction via GPx1/ROS/KCa3.1 Signal Axis in Diabetes
Frontiers in Cell and Developmental Biology, Mar 12, 2021; DOI: 10.3389/fcell.2021.651434
Mouse ileal organoid lines
Author: Brendan Dolan
Date: Oct 3, 2022，Views：330
Original protocol used in research article：
Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport
Science Signaling, Sep 20, 2022; DOI: 10.1126/scisignal.abl5848
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