Bio-101 - Improve Research Reproducibility

Flow cytometry–based ICAM1-binding assay

Authors: Kristoffer H. Johansen, Klaus Okkenhaug and Pamela L. Schwartzberg
Date: Sep 21, 2022，Views：611

Original protocol used in research article：

A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1–mediated adhesion in T cells
Science Signaling, Jul 19, 2022; DOI: 10.1126/scisignal.abl9169

Supporting materials for "Standardized and reproducible measurement of decision-making in mice"

Author: International Brain Laboratory
Date: Sep 21, 2022，Views：602

Original protocol used in research article：

Standardized and reproducible measurement of decision-making in mice
eLife, May 20, 2021; DOI: 10.7554/eLife.63711

Cell adhesion assay

Authors: Bochuan Li, Ying Yang, Hongfeng Jiang and Degang Liang
Date: Sep 20, 2022，Views：608

Original protocol used in research article：

RNA N⁶-methyladenosine modulates endothelial atherogenic responses to disturbed flow in mice
eLife, Jan 10, 2022; DOI: 10.7554/eLife.69906

Immunofluorescence

Authors: Kristine Schauer, Mathieu Coppey and Kotryna Vaidziulyte
Date: Sep 20, 2022，Views：528

Original protocol used in research article：

Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking
eLife, Mar 18, 2022; DOI: 10.7554/eLife.69229

Primary hair cell culture

Authors: Louise Menendez, Justin Ichida and Neil Segil
Date: Sep 19, 2022，Views：545

Original protocol used in research article：

Generation of inner ear hair cells by direct lineage conversion of primary somatic cells
eLife, Jun 30, 2020; DOI: 10.7554/eLife.55249

Immuno-fluorescent (IF) staining

Authors: Minyan Zheng and Veronica F Hinman
Date: Sep 19, 2022，Views：537

Original protocol used in research article：

Regeneration of the larval sea star nervous system by wounding induced respecification to the Sox2 lineage
eLife, Jan 14, 2022; DOI: 10.7554/eLife.72983

Exploration classifier details

Authors: Zachary Zeidler and Laura A DeNardo
Date: Sep 19, 2022，Views：542

Original protocol used in research article：

BehaviorDEPOT is a simple, flexible tool for automated behavioral detection based on markerless pose tracking
eLife, ; DOI: 10.7554/eLife.74314

Exploration classifier details

Authors: Zachary Zeidler and Laura A DeNardo
Date: Sep 19, 2022，Views：540

Original protocol used in research article：

BehaviorDEPOT is a simple, flexible tool for automated behavioral detection based on markerless pose tracking
eLife, ; DOI: 10.7554/eLife.74314

DNA extraction and 16S amplicon sequencing

Authors: Steven L. Taylor and Geraint B. Rogers
Date: Sep 18, 2022，Views：538

Original protocol used in research article：

Ear microbiota and middle ear disease: a longitudinal pilot study of Aboriginal children in a remote south Australian setting
BMC Microbiology, Jan 13, 2022; DOI: 10.1186/s12866-022-02436-x

TcGDT1-3xHA overexpression

Authors: Srinivasan Ramakrishnan and Roberto Docampo
Date: Sep 17, 2022，Views：557

Original protocol used in research article：

Deletion of a Golgi protein in Trypanosoma cruzi reveals a critical role for Mn2+ in protein glycosylation needed for host cell invasion and intracellular replication
PLoS Pathogens, Mar 15, 2021; DOI: 10.1371/journal.ppat.1009399

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