Protocols shared by authors via RaP service
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Authors: Nak Hyun Kim, Zhen-Ming Pei, Li Wan and Jeffery L. Dangl
Date: Sep 27, 2022，Views：555
Original protocol used in research article：
Plant “helper” immune receptors are Ca2+-permeable nonselective cation channels
Science, Jul 23, 2021; DOI: 10.1126/science.abg7917
Designing and cloning of the gRNA
Authors: Kumarasamypet M Mohankumar and Nithin Sam R
Date: Sep 27, 2022，Views：544
Original protocol used in research article：
Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin
eLife, Feb 11, 2022; DOI: 10.7554/eLife.65421
Preparation of single-cell suspensions
Author: Claire E. O’Leary
Date: Sep 26, 2022，Views：531
Original protocol used in research article：
Bile acid–sensitive tuft cells regulate biliary neutrophil influx
Science Immunology, Mar 4, 2022; DOI: 10.1126/sciimmunol.abj1080
Lentivirus infection of organoids to test infection efficiency
Authors: Dawei Sun and Emma L Rawlins
Date: Sep 26, 2022，Views：663
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
LC-MS/MS sample preparation
Authors: Jonathan G Van Vranken and Steven P Gygi
Date: Sep 26, 2022，Views：606
Original protocol used in research article：
Assessing target engagement using proteome-wide solvent shift assays
eLife, Dec 8, 2021; DOI: 10.7554/eLife.70784
In vitro pull-down assay
Authors: Yue Xiong and Yan-ping Xu
Date: Sep 25, 2022，Views：555
Original protocol used in research article：
USP15 suppresses tumor immunity via deubiquitylation and inactivation of TET2
Science Advances, Sep 17, 2020; DOI: 10.1126/sciadv.abc9730
Competitive fitness assays
Authors: Hala Tamim El Jarkass and Aaron W Reinke
Date: Sep 23, 2022，Views：528
Original protocol used in research article：
An intestinally secreted host factor promotes microsporidia invasion of C. elegans
eLife, Jan 7, 2022; DOI: 10.7554/eLife.72458
ASC Speck Quantification and High Magnification Imaging
Authors: Hayley I. Muendlein and Alexander Poltorak
Date: Sep 22, 2022，Views：530
Original protocol used in research article：
cFLIPL protects macrophages from LPS-induced pyroptosis via inhibition of complex II formation
Science, Mar 19, 2020; DOI: 10.1126/science.aay3878
Fabrication of ORMs and catalase conjugation
Authors: Ya Guan, Hong Niu and Jianjun Guan
Date: Sep 22, 2022，Views：537
Original protocol used in research article：
Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
Science Advances, Aug 27, 2021; DOI: 10.1126/sciadv.abj0153
Synthesis and characterization of ROS-scavenging hydrogel
Authors: Ya Guan, Hong Niu and Jianjun Guan
Date: Sep 22, 2022，Views：551
Original protocol used in research article：
Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
Science Advances, Aug 27, 2021; DOI: 10.1126/sciadv.abj0153
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