Protocols shared by authors via RaP service
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Determination of protein copy number per cell
Authors: Rogelio A. Hernandez-Lopez and Wendell A. Lim
Date: Oct 16, 2022，Views：355
Original protocol used in research article：
T cell circuits that sense antigen density with an ultrasensitive threshold
Science, Mar 12, 2021; DOI: 10.1126/science.abc1855
Chromatin IP with reference exogenous genome
Authors: Zhenning Wang, Aixia Song and Fei Xavier Chen
Date: Oct 16, 2022，Views：303
Original protocol used in research article：
Coordinated regulation of RNA polymerase II pausing and elongation progression by PAF1
Science Advances, Apr 1, 2022; DOI: 10.1126/sciadv.abm5504
PP1 Phosphatase assay
Author: Ayse Koca Caydasi
Date: Oct 14, 2022，Views：394
Original protocol used in research article：
Protein phosphatase 1 in association with Bud14 inhibits mitotic exit in Saccharomyces cerevisiae
eLife, Oct 11, 2021; DOI: 10.7554/eLife.72833
Protein analysis by immunoblotting
Authors: Manoj Arra and Yousef Abu-Amer
Date: Oct 13, 2022，Views：261
Original protocol used in research article：
Glutamine metabolism modulates chondrocyte inflammatory response
eLife, Aug 2, 2022; DOI: 10.7554/eLife.80725
Quantification of fungal growth by qPCR
Author: Shouan Liu
Date: Oct 12, 2022，Views：337
Original protocol used in research article：
Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100
eLife, Jun 15, 2015; DOI: 10.7554/eLife.07295
Single animal PCR genotyping protocol for C. elegans
Authors: Jian Chen and Tim Schedl
Date: Oct 10, 2022，Views：533
Original protocol used in research article：
A simple one-step PCR assay for SNP detection
microPublication Biology, Jun 11, 2021; DOI: 10.17912/micropub.biology.000399
Dual-Luciferase Transient Expression Assays of Arabidopsis Protoplasts
Authors: Jun Zhu and Zhong-Nan Yang
Date: Oct 9, 2022，Views：479
Original protocol used in research article：
OsMS188 Is a Key Regulator of Tapetum Development and Sporopollenin Synthesis in Rice
Rice, Jan 6, 2021; DOI: 10.1186/s12284-020-00451-y
In-home radio-based gait monitoring
Author: Yingcheng Liu
Date: Oct 7, 2022，Views：391
Original protocol used in research article：
Monitoring gait at home with radio waves in Parkinson’s disease: A marker of severity, progression, and medication response
Science Translational Medicine, Sep 21, 2022; DOI: 10.1126/scitranslmed.adc9669
Hi-C data processing and A/B compartment calculation
Authors: Kei Fukuda and Yoichi Shinkai
Date: Oct 6, 2022，Views：362
Original protocol used in research article：
Regulation of mammalian 3D genome organization and histone H3K9 dimethylation by H3K9 methyltransferases
Communications Biology, May 13, 2021; DOI: 10.1038/s42003-021-02089-y
Inducing blunt force trauma in third-instar Drosophila melanogaster larvae using the High-Impact Trauma (HIT) device
Authors: Jorgo Lika, Rebeccah J Katzenberger, Barry Ganetzky and David A Wassarman
Date: Oct 6, 2022，Views：284
Original protocol used in research article：
Effects of blunt force injuries in third-instar Drosophila larvae persist through metamorphosis and reduce adult lifespan
microPublication Biology, ; DOI: 10.17912/micropub.biology.000423
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