Bio-101 - Improve Research Reproducibility

Ex vivo lipolysis

Authors: Ziru Li and Ormond A MacDougald
Date: Feb 15, 2023，Views：463

Original protocol used in research article：

Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496

Instructions on How to Make the Mason Lab Rat Restrainers

Author: Peggy Mason
Date: Feb 4, 2023，Views：482

Original protocol used in research article：

Pro-social behavior in rats is modulated by social experience
eLife, Jan 14, 2014; DOI: 10.7554/eLife.01385

Antibodies and immunocytochemistry

Authors: Christophe P Ribelayga and Stephen C Massey
Date: Feb 1, 2023，Views：479

Original protocol used in research article：

Analysis of rod/cone gap junctions from the reconstruction of mouse photoreceptor terminals
eLife, ; DOI: 10.7554/eLife.73039

Subcellular fractionation and RNA extraction

Authors: Ke-En Tan, Chee-Kwee Ea and Yat-Yuen Lim
Date: Jan 30, 2023，Views：495

Original protocol used in research article：

Identification and characterization of a novel Epstein-Barr Virus-encoded circular RNA from LMP-2 Gene
Scientific Reports, Jul 13, 2021; DOI: 10.1038/s41598-021-93781-w

Secretome Collection

Authors: Hui MA, Ping-Kuen Lam, Wing-Sum Siu and Ping-Chung Leung
Date: Jan 30, 2023，Views：523

Original protocol used in research article：

Adipose Tissue-Derived Mesenchymal Stem Cells (ADMSCs) and ADMSC-Derived Secretome Expedited Wound Healing in a Rodent Model – A Preliminary Study
Clinical, Cosmetic and Investigational Dermatology, Jun 30, 2021; DOI: 10.2147/CCID.S298105

Preparation of CAR T cells in fibrin gel

Authors: Ugur Uslu and Carl H. June
Date: Jan 26, 2023，Views：518

Original protocol used in research article：

Chimeric antigen receptor T cells as adjuvant therapy for unresectable adenocarcinoma
Science Advances, Jan 11, 2023; DOI: 10.1126/sciadv.ade2526

3.3. CUPRAC Assay (Spectrophotometry)

Authors: Xican Li and Hong Xie
Date: Jan 25, 2023，Views：508

Original protocol used in research article：

Effect of Double Bond Position on 2-Phenyl-benzofuran Antioxidants: A Comparative Study of Moracin C and Iso-Moracin C
Molecules, Mar 24, 2018; DOI: 10.3390/molecules23040754

SELEX Library Construction.

Authors: Ryo Amano, Yoshikazu Nakamura and Masaki Takahashi
Date: Jan 24, 2023，Views：558

Original protocol used in research article：

Nucleic acid ligands act as a PAM and agonist depending on the intrinsic ligand binding state of P2RY2
Proceedings of the National Academy of Sciences of the United States of America, Apr 28, 2021; DOI: 10.1073/pnas.2019497118

Mono-allelic expression of HLA-I alleles for HLA-peptidomics analysis

Authors: Adi Nagler, Shelly Kalaora, Chaya Barbolin, Michal Alon, Polina Greenberg, Gal Yagel, Aviyah Peri, Yishai Levin and Yardena Samuels
Date: Jan 24, 2023，Views：541

Original protocol used in research article：

Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics
Cell Rep, Jun 8, 2021; DOI: 10.1016/j.celrep.2021.109305

TERRA RNA-FISH

Authors: Rosa María Marión and Maria A Blasco
Date: Jan 23, 2023，Views：524

Original protocol used in research article：

TERRA regulate the transcriptional landscape of pluripotent cells through TRF1-dependent recruitment of PRC2
eLife, Aug 20, 2019; DOI: 10.7554/eLife.44656

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