Protocols shared by authors via RaP service
Journal
Adipocyte nuclei isolation from iWAT
Author: Prashant Rajbhandari
Date: Feb 24, 2020，Views：2310
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
Assembly of stalled yeast Pol II transcription elongation complex
Authors: Zhijie Chen, Ronen Gabizon, Tingting Yao and Carlos Bustamante
Date: Feb 23, 2020，Views：1536
Original protocol used in research article：
High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier
eLife, Jul 31, 2019; DOI: 10.7554/eLife.48281
Subcellular fractionation
Author: Rosa Puertollano
Date: Feb 21, 2020，Views：1440
Original protocol used in research article：
The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damage
eLife, Dec 6, 2018; DOI: 10.7554/eLife.40856
Primary NSC Culture
Authors: Isabelle Maria Blomfield and François Guillemot
Date: Feb 21, 2020，Views：1536
Original protocol used in research article：
Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells
eLife, Sep 25, 2019; DOI: 10.7554/eLife.48561
Juxtacellular labelling of single neurons
Author: Navid Farassat
Date: Feb 21, 2020，Views：1317
Original protocol used in research article：
In vivo functional diversity of midbrain dopamine neurons within identified axonal projections
eLife, Oct 3, 2019; DOI: 10.7554/eLife.48408
Chromatin immunoprecipitation (ChIP)
Authors: Xuehua Zhong and Xiangsong Chen
Date: Feb 20, 2020，Views：1488
Original protocol used in research article：
POWERDRESS interacts with HISTONE DEACETYLASE 9 to promote aging in Arabidopsis
eLife, Nov 22, 2016; DOI: 10.7554/eLife.17214
Automatic determination of optimal cluster number
Author: Jakob Nikolas Kather
Date: Feb 20, 2020，Views：1377
Original protocol used in research article：
Topography of cancer-associated immune cells in human solid tumors
eLife, Sep 4, 2018; DOI: 10.7554/eLife.36967
α-syn purification
Author: Smriti Sangwan
Date: Feb 20, 2020，Views：1324
Original protocol used in research article：
Inhibition of synucleinopathic seeding by rationally designed inhibitors
eLife, Jan 2, 2020; DOI: 10.7554/eLife.46775
mRNA preparation
Author: Maaike Welling
Date: Feb 20, 2020，Views：3761
Original protocol used in research article：
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
eLife, Jan 21, 2019; DOI: 10.7554/eLife.44491
In vitro kinase assay using immunoprecipitated Cdk5 from SCN
Authors: Andrea Brenna and Urs Albrecht
Date: Feb 20, 2020，Views：1390
Original protocol used in research article：
Cyclin-dependent kinase 5 (CDK5) regulates the circadian clock
eLife, Nov 5, 2019; DOI: 10.7554/eLife.50925
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