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A high-quality database for basic life science protocols
Protocols
Research fields
Biochemistry (1376)
Biophysics (164)
Cancer Biology (431)
Cell Biology (2122)
Developmental Biology (321)
Immunology (597)
Microbiology (1154)
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Immunofluorescence assay for 5hmc
Author:
Vladimir Camarena
Date: Nov 18, 2019，Views：1442
Original protocol used in research article：
cAMP signaling regulates DNA hydroxymethylation by augmenting the intracellular labile ferrous iron pool
eLife, Dec 14, 2017; DOI:
10.7554/eLife.29750
GFP dropout screening
Author:
Jason Sheltzer
Date: Nov 18, 2019，Views：1475
Original protocol used in research article：
CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going clinical trials
eLife, Mar 24, 2017; DOI:
10.7554/eLife.24179
Rotarod (Ugo Basile Biological Research Apparatus)
Author:
Cristian A Lasagna-Reeves
Date: Nov 18, 2019，Views：2025
Original protocol used in research article：
Ataxin-1 oligomers induce local spread of pathology and decreasing them by passive immunization slows Spinocerebellar ataxia type 1 phenotypes
eLife, Dec 17, 2015; DOI:
10.7554/eLife.10891
Detailed protocol for mouse adipose tissue protein extraction
Authors:
Yu An
and Philipp E Scherer
Date: Nov 18, 2019，Views：4650
Original protocol used in research article：
Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis
eLife, Mar 29, 2017; DOI:
10.7554/eLife.24071
Native-PAGE to study BiP oligomeric complexes
Author:
Steffen Preissler
Date: Nov 18, 2019，Views：6937
Original protocol used in research article：
AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
eLife, Dec 17, 2015; DOI:
10.7554/eLife.12621
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