Bio-101 - Improve Research Reproducibility

Viral Polymerase Activity Assay

Authors: Lianzhong Zhao and Meilin Jin
Date: Jul 4, 2021，Views：778

Original protocol used in research article：

Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins
Frontiers in Microbiology, Oct 15, 2020; DOI: 10.3389/fmicb.2020.538355

Detection of phospho-epitopes on human NK cells for cell signaling analysis

Authors: Niklas K. Björkström and Martin Cornillet
Date: Jul 4, 2021，Views：809

Original protocol used in research article：

NK cells are activated and primed for skin-homing during acute dengue virus infection in humans
Nature Communications, Aug 29, 2019; DOI: 10.1038/s41467-019-11878-3

Spontaneous and lineage-directed differentiation of hESCs and hiPSCs

Authors: Jiwon Jang and Kenneth S. Kosik
Date: Jul 3, 2021，Views：787

Original protocol used in research article：

Control over single-cell distribution of G1 lengths by WNT governs pluripotency
PLoS Biology, Sep 26, 2019; DOI: 10.1371/journal.pbio.3000453

2.14. Isolation of Primary Liver Sinusoidal Endothelial Cells

Author: Hannah K. Drescher
Date: Jul 2, 2021，Views：819

Original protocol used in research article：

L-Selectin/CD62L Is a Key Driver of Non-Alcoholic Steatohepatitis in Mice and Men
Cells, Apr 29, 2020; DOI: 10.3390/cells9051106

Preparation of libraries for yeast ribosome footprints

Authors: Rachel Green and Colin Wu
Date: Jul 2, 2021，Views：817

Original protocol used in research article：

The endonuclease Cue2 cleaves mRNAs at stalled ribosomes during No Go Decay
eLife, Jun 20, 2019; DOI: 10.7554/eLife.49117

Rhodamine 6G efflux assay

Authors: Bettina C. Fries and Somanon Bhattacharya
Date: Jul 2, 2021，Views：814

Original protocol used in research article：

Gene Duplication Associated with Increased Fluconazole Tolerance in Candida auris cells of Advanced Generational Age
Scientific Reports, Mar 25, 2019; DOI: 10.1038/s41598-019-41513-6

2.13. Immunofluorescence Staining of the Kidney Sections

Authors: Gry H. Dihazi, Marwa Eltoweissy, Olaf Jahn and Hassan Dihazi
Date: Jul 2, 2021，Views：742

Original protocol used in research article：

The Secretome Analysis of Activated Human Renal Fibroblasts Revealed Beneficial Effect of the Modulation of the Secreted Peptidyl-Prolyl Cis-Trans Isomerase A in Kidney Fibrosis
Cells, Jan 1, 2020; DOI: 10.3390/cells9071724

Enzymatic Method Measurement of Serum Amylase and Lipase

Authors: Shengchuan Chen and Feng Ma
Date: Jul 2, 2021，Views：808

Original protocol used in research article：

TLR3 Ligand PolyI:C Prevents Acute Pancreatitis Through the Interferon-β/Interferon-α/β Receptor Signaling Pathway in a Caerulein-Induced Pancreatitis Mouse Model
Frontiers in Immunology, May 3, 2019; DOI: 10.3389/fimmu.2019.00980

Time-lapse live imaging.

Authors: Allon M. Klein and Ophir D. Klein
Date: Jul 1, 2021，Views：752

Original protocol used in research article：

A large pool of actively cycling progenitors orchestrates self-renewal and injury repair of an ectodermal appendage
Nat Cell Biol, Sep 2, 2019; DOI: 10.1038/s41556-019-0378-2

In vitro kinase assays for STPKs.

Author: Ramandeep Singh
Date: Jun 30, 2021，Views：759

Original protocol used in research article：

NU-6027 Inhibits Growth of Mycobacterium tuberculosis by Targeting Protein Kinase D and Protein Kinase G
Antimicrobial Agents and Chemotherapy, Aug 23, 2019; DOI: 10.1128/AAC.00996-19

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