细胞生物学

分类

    现刊
    Acute Cerebellar Slice Preparation Using a Tissue Chopper
    使用组织切碎机快速制备小脑切片
    作者:Yubin Wang and Michel Baudry日期:03/05/2019,浏览量:869,Q&A: 0
    [Abstract] Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
    Identification and Quantitation of Leukocyte Populations in Human Kidney Tissue by Multi-parameter Flow Cytometry
    利用多参数流式细胞技术对人肾脏组织中白细胞种群的鉴定与定量分析
    作者:Katrina Kildey, Becker M.P. Law, Kimberly A. Muczynski, Ray Wilkinson, Helen Healy and Andrew J. Kassianos日期:08/20/2018,浏览量:1989,Q&A: 0
    [Abstract] Inflammatory immune cells play direct pathological roles in cases of acute kidney injury (AKI) and chronic kidney disease (CKD). However, the identification and characterization of distinct populations of leukocytes in human kidney biopsies have been confounded by the limitations of immunohistochemical (IHC)-based techniques used to detect them. ...
    In vitro Explant Cultures to Interrogate Signaling Pathways that Regulate Mouse Lung Development
    探寻调控小鼠肺部发育的信号转导途径的体外外植体培养
    作者:Changfu Yao, Gianni Carraro and Barry R. Stripp日期:05/20/2018,浏览量:2346,Q&A: 0
    [Abstract] Early mouse lung development, including specification of primordia, patterning of early endoderm and determination of regional progenitor cell fates, is tightly regulated. The ability to culture explanted embryonic lung tissue provides a tractable model to study cellular interactions and paracrine factors that regulate these processes. We provide ...
    Organotypic Explants of the Embryonic Rodent Hippocampus: An Accessible System for Transgenesis
    啮齿动物胚胎海马器官型外植体:一个可进行转基因的系统
    作者:Archana Iyer , Lakshmi Subramanian and Shubha Tole日期:03/20/2018,浏览量:2523,Q&A: 0
    [Abstract] This protocol describes the technique of ex-vivo electroporation to target embryonic hippocampal progenitors in an organotypic slice preparation. This technique allows gene perturbation for examining developmental processes in the embryonic hippocampus while retaining the environment and connectivity of the cells. Gene perturbation can ...
    Obtaining Acute Brain Slices
    急性脑组织切片的制备
    作者:Thomas Papouin and Philip G. Haydon日期:01/20/2018,浏览量:6498,Q&A: 1
    [Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
    Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery
    使用慢病毒递送的离体滋养层特定基因操作
    作者:Damayanti Chakraborty, Masanaga Muto and Michael J. Soares日期:12/20/2017,浏览量:3057,Q&A: 0
    [Abstract] In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of ‘gain-of-function’ and ‘loss-of-function’ blastocyst ...
    Mandibular Explant Assay for Investigating Extrinsic Stimuli on Bone and Cartilage Development
    研究骨及软骨外源刺激的下颌外植体实验
    作者:Jamie Jiang, Jessica W. Bertol and Walid D. Fakhouri日期:12/05/2017,浏览量:3316,Q&A: 0
    [Abstract] A major issue in developmental biology is to determine how embryonic tissues respond to molecular signals in a timely manner and given the position-restricted instructions during morphogenesis, of which Meckel’s cartilage is a classical example. The ex-vivo explant model is a practical and convenient system that allows investigation of ...
    Quantification of Trypanosoma cruzi in Tissue and Trypanosoma cruzi Killing Assay
    组织中克氏锥虫的定量及克氏锥虫杀灭试验
    作者:Hisako Kayama, Shoko Kitada and Kiyoshi Takeda日期:11/20/2017,浏览量:3516,Q&A: 0
    [Abstract] Infection with Trypanosoma cruzi causes Chagas disease. The methods provided here allow for the quantification of T. cruzi in the liver, heart, and blood of intraperitoneally-infected mice and analysis of the killing activity of the cells infected with T. cruzi in vitro.
    Preparation of Teased Nerve Fibers from Rat Sciatic Nerve
    利用大鼠坐骨神经分离制备单根神经纤维
    作者:Jinkun Wen, Lixia Li, Dandan Tan and Jiasong Guo日期:10/05/2017,浏览量:4629,Q&A: 0
    [Abstract] Compared to tissue sectioning techniques, the technique of teasing single nerve fibers provides a better way to understand the structures of myelin sheaths and axons of the peripheral myelinated nerves. This protocol describes a method for preparation of teased single nerve fibers from rat sciatic nerve. In this protocol, fixed nerves are teased ...
    Isolation of Rodent Brain Vessels
    啮齿动物脑血管分离
    作者:Cristina Carvalho and Paula I. Moreira日期:09/05/2017,浏览量:4078,Q&A: 0
    [Abstract] The prevalence of neurodegenerative diseases is increasing worldwide. Cerebrovascular disorders and/or conditions known to affect brain vasculature, such as diabetes, are well-known risk factors for neurodegenerative diseases. Thus, the evaluation of the brain vasculature is of great importance to better understand the mechanisms underlying brain ...