Protocols shared by authors via RaP service
Journal
Human erythrocyte hemolysis assay
Authors: Aftab Nadeem and Sun Nyunt Wai
Date: Sep 6, 2022，Views：624
Original protocol used in research article：
Protein-lipid interaction at low pH induces oligomerization of the MakA cytotoxin from Vibrio cholerae
eLife, Feb 8, 2022; DOI: 10.7554/eLife.73439
Covariates for GLM
Authors: Katy AM Gaythorpe and Neil Ferguson
Date: Sep 5, 2022，Views：539
Original protocol used in research article：
The global burden of yellow fever
eLife, Mar 16, 2021; DOI: 10.7554/eLife.64670
KCC2 measurements and analysis of subcellular distribution
Authors: Franck Aby and Pascal Fossat
Date: Sep 5, 2022，Views：550
Original protocol used in research article：
Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain
Science Advances, Jul 27, 2022; DOI: 10.1126/sciadv.abo0689
Immunofluorescence
Authors: Jeffrey Lee, Peter Wing, Alfredo Castello, Jane A McKeating and Ilan Davis
Date: Sep 5, 2022，Views：581
Original protocol used in research article：
Absolute quantitation of individual SARS-CoV-2 RNA molecules provides a new paradigm for infection dynamics and variant differences
eLife, Jan 20, 2022; DOI: 10.7554/eLife.74153
Isolation by FACS sorting for qRT-PCR of Drosophila larvae class IV da neurons
Authors: Jacob Jaszczak, Laura Devault, Lily Jan and Yuh Nung Jan
Date: Sep 2, 2022，Views：554
Original protocol used in research article：
Steroid hormone signaling activates thermal nociception during Drosophila peripheral nervous system development
eLife, Mar 30, 2022; DOI: 10.7554/eLife.76464
Protein interaction analysis in Y2H system
Authors: Vladimir Denic and Houqing Yu
Date: Sep 1, 2022，Views：698
Original protocol used in research article：
The peroxisomal exportomer directly inhibits phosphoactivation of the pexophagy receptor Atg36 to suppress pexophagy in yeast
eLife, Apr 11, 2022; DOI: 10.7554/eLife.74531
In vitro kinase assay
Authors: Vladimir Denic and Houqing Yu
Date: Sep 1, 2022，Views：1126
Original protocol used in research article：
The peroxisomal exportomer directly inhibits phosphoactivation of the pexophagy receptor Atg36 to suppress pexophagy in yeast
eLife, Apr 11, 2022; DOI: 10.7554/eLife.74531
Adapter ligation and RT-PCR
Authors: Emil Laust Kristoffersen, Agnes Noy and Philipp Holliger
Date: Sep 1, 2022，Views：689
Original protocol used in research article：
Rolling circle RNA synthesis catalyzed by RNA
eLife, Feb 2, 2022; DOI: 10.7554/eLife.75186
mib1_ta52b 4 primer genotyping protocol
Author: Maximilian Fürthauer
Date: Sep 1, 2022，Views：531
Original protocol used in research article：
The E3 ubiquitin ligase mindbomb1 controls planar cell polarity-dependent convergent extension movements during zebrafish gastrulation
eLife, Feb 10, 2022; DOI: 10.7554/eLife.71928
FAK purification
Authors: Lindsay B Case and Michael K Rosen
Date: Aug 31, 2022，Views：544
Original protocol used in research article：
Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation
eLife, Jan 20, 2022; DOI: 10.7554/eLife.72588
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