细胞生物学

分类

    现刊
    Insect Feeding Assays with Spodoptera exigua on Arabidopsis thaliana
    甜菜夜蛾对拟南芥的取食试验
    作者:Yanrong You, Chunpeng An and Chuanyou Li日期:03/05/2020,浏览量:1240,Q&A: 0
    [Abstract] Plant-insect interaction is an important field for studying plant immunity. The beet armyworm, Spodoptera exigua, is one of the best-known agricultural pest insects and is usually used to study plant interactions with chewing insects. Here, we describe a protocol for insect feeding assays with Spodoptera exigua lavae using model ...
    Using Arabidopsis Mesophyll Protoplasts to Study Unfolded Protein Response Signaling
    利用拟南芥叶肉细胞原生质体研究未折叠蛋白响应信号
    作者:Yan Bao and Diane C. Bassham日期:12/05/2018,浏览量:3094,Q&A: 0
    [Abstract] Various environmental stresses or artificial reagents can trigger unfolded protein accumulation in the endoplasmic reticulum (ER) due to the folding capacity of the ER being exceeded. This is termed ER stress, and triggers the unfolded protein response (UPR). Assays for activation of the UPR in plants include Tunicamycin (Tm)- or dithiothreitol ...
    Determination of Reduced and Total Glutathione Content in Extremophilic Microalga Galdieria phlegrea
    极端环境微藻Galdieria phlegrea中还原型和总谷胱甘肽含量的测定
    作者:Giovanna Salbitani, Claudia Bottone and Simona Carfagna日期:07/05/2017,浏览量:12996,Q&A: 2
    [Abstract] Glutathione is an important molecule involved in the primary and secondary metabolism of all organisms. The Glutathione redox status is an indicator of the cellular redox state. Therefore, it is important to have precise methods on hand to determine the glutathione redox status in the cell. In this protocol, we describe an improved ...
    P-body and Stress Granule Quantification in Caenorhabditis elegans
    秀丽隐杆线虫中P-body和应激颗粒的定量
    作者:Matthias Rieckher and Nektarios Tavernarakis日期:01/20/2017,浏览量:8673,Q&A: 0
    [Abstract] Eukaryotic cells contain various types of cytoplasmic, non-membrane bound ribonucleoprotein (RNP) granules that consist of non-translating mRNAs and a versatile set of associated proteins. One prominent type of RNP granules is Processing bodies (P bodies), which majorly harbors translationally inactive mRNAs and an array of proteins mediating mRNA ...
    In vitro Treatment of Mouse and Human Cells with Endogenous Ligands for Activation of the Aryl Hydrocarbon Receptor
    使用内源性配体体外处理小鼠和人类细胞激活芳烃受体
    作者:Taisho Yamada and Akinori Takaoka日期:01/05/2017,浏览量:5406,Q&A: 0
    [Abstract] Activation of the aryl hydrocarbon receptor (AHR) by endogenous ligands has been implicated in a variety of physiological processes such as cell cycle regulation, cell differentiation and immune responses. It is reported that tryptophan metabolites, such as kynurenine (Kyn) and 6-formylindolo(3,2-b)carbazole (FICZ), are endogenous ligands for AHR ...
    FICZ Exposure and Viral Infection in Mice
    小鼠FICZ暴露与病毒感染
    作者:Taisho Yamada and Akinori Takaoka日期:01/05/2017,浏览量:5650,Q&A: 0
    [Abstract] The aryl hydrocarbon receptor (AHR) is known as a sensor for dioxins that mediates their toxicity, and also has important biophysiological roles such as circadian rhythms, cell differentiation and immune responses. 6-formylindolo(3,2-b)carbazole (FICZ), which is derived through the metabolism of L-tryptophan by ultraviolet B irradiation, is one of ...
    In vivo Analysis of Neutrophil Infiltration during LPS-induced Peritonitis
    LPS诱导腹膜炎过程中嗜中性粒细胞免疫浸润分析
    作者:Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik日期:10/05/2016,浏览量:7175,Q&A: 0
    [Abstract] Bacterial lipopolysaccharide (LPS) is present in the outer membrane of Gram-negative bacteria and functions as pathogen-associated molecular pattern (PAMP) (Whitfield and Trent, 2014). LPS therefore is a potent activator of inflammatory responses leading to cytokine release and neutrophils recruitment. The lipid A moiety of LPS activates the ...
    ASC-particle-induced Peritonitis
    ASC颗粒诱导腹膜炎模型的构建
    作者:Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik日期:10/05/2016,浏览量:4688,Q&A: 0
    [Abstract] In response to pathogen infection and tissue damage, inflammasome sensors such as NLRP3 and AIM2 are activated, which triggers PYRIN domain (PYD)-mediated ASC nucleation, followed by self-perpetuating ASC polymerization, which ultimately culminates in caspase-1 activation, interleukin (IL)-1β and IL-18 processing and release and pyroptosis ...
    Hepatitis C virus Cell-to-cell Spread Assay
    丙型肝炎病毒在细胞之间的传播试验
    作者:Naina Barretto and Susan L. Uprichard日期:12/20/2014,浏览量:8162,Q&A: 1
    [Abstract] Hepatitis C virus (HCV) can infect naïve cells via entry of “cell-free” extracellular virus or direct “cell-to-cell” transmission. Here, we describe an assay for detecting HCV cell-to-cell transmission, using a non-growing cell culture system that avoids confounding effects of cell growth. The assay consists of infecting a small number of cells in ...
    Signaling Assays for Detection of Human G-protein-coupled Receptors in Yeast
    以酵母为模型对人G蛋白偶联受体进行信号传导分析
    作者:Yasuyuki Nakamura, Jun Ishii and Akihiko Kondo日期:08/20/2014,浏览量:8197,Q&A: 0
    [Abstract] G-protein-coupled receptors (GPCRs) are the largest group of cell-surface proteins and are major molecular targets for drug development. The protocol described herein is for the detection of human GPCR signaling in the yeast Saccharomyces cerevisiae. Using Zoanthus sp. green fluorescent protein (ZsGreen) as the reporter, ...