Protocols shared by authors via RaP service
Journal
Materials and antibodies
Authors: MW Khan and P Chakrabarti
Date: Nov 19, 2022，Views：981
Original protocol used in research article：
mTORC2 controls cancer cell survival by modulating gluconeogenesis
Cell Death Discovery, Sep 7, 2015; DOI: 10.1038/cddiscovery.2015.16
2.7.4. Synthesis of Acid chloroauric trihydrate (HAuCl4.3H2O)
Authors: Nestor Sanchez and Martha Cobo
Date: Nov 17, 2022，Views：2642
Original protocol used in research article：
Life cycle inventory data for power production from sugarcane press-mud
Data in Brief, Jun 3, 2021; DOI: 10.1016/j.dib.2021.107194
V2R expression and purification
Authors: Julien Bous, Sébastien Granier and Bernard Mouillac
Date: Nov 17, 2022，Views：943
Original protocol used in research article：
Cryo–electron microscopy structure of the antidiuretic hormone arginine-vasopressin V2 receptor signaling complex
Science Advances, May 21, 2021; DOI: 10.1126/sciadv.abg5628
Ex vivo simulated ischemia reperfusion
Authors: Gregory A. Wyant and William G. Kaelin
Date: Nov 16, 2022，Views：1008
Original protocol used in research article：
Mitochondrial remodeling and ischemic protection by G protein–coupled receptor 35 agonists
Science, Aug 4, 2022; DOI: 10.1126/science.abm1638
Isolation of renal-infiltrating T cells
Authors: Ping-Min Chen and Joe Craft
Date: Nov 15, 2022，Views：935
Original protocol used in research article：
Kidney tissue hypoxia dictates T cell–mediated injury in murine lupus nephritis
Science Translational Medicine, Apr 7, 2020; DOI: 10.1126/scitranslmed.aay1620
Cell culture and reagents
Authors: Yool Lee and Amita Sehgal
Date: Nov 15, 2022，Views：565
Original protocol used in research article：
Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abd2645
Real-time reverse transcription PCR
Author: Xintao Shuai
Date: Nov 14, 2022，Views：841
Original protocol used in research article：
Dual pH-sensitive nanodrug blocks PD-1 immune checkpoint and uses T cells to deliver NF-κB inhibitor for antitumor immunotherapy
Science Advances, Feb 4, 2020; DOI: 10.1126/sciadv.aay7785
Code availability
Authors: DongAhn Yoo, Jeremy S Dasen, Heebal Kim and Myungin Baek
Date: Nov 8, 2022，Views：602
Original protocol used in research article：
Little skate genome provides insights into genetic programs essential for limb-based locomotion
eLife, Oct 26, 2022; DOI: 10.7554/eLife.78345
Detection of phosphoepitopes by Flow cytometry
Authors: Antoine Marçais and Thierry Walzer
Date: Nov 7, 2022，Views：746
Original protocol used in research article：
High mTOR activity is a hallmark of reactive natural killer cells and amplifies early signaling through activating receptors
eLife, Sep 6, 2017; DOI: 10.7554/eLife.26423
ENCODE dynamic k-mer–assisted mapping
Authors: Ariel Gershman, Michael E. G. Sauria, Karen H. Miga and Winston Timp
Date: Nov 7, 2022，Views：622
Original protocol used in research article：
Epigenetic patterns in a complete human genome
Science, Apr 1, 2022; DOI: 10.1126/science.abj5089
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