Bio-101 - Improve Research Reproducibility

Exploration classifier details

Authors: Zachary Zeidler and Laura A DeNardo
Date: Sep 19, 2022，Views：583

Original protocol used in research article：

BehaviorDEPOT is a simple, flexible tool for automated behavioral detection based on markerless pose tracking
eLife, ; DOI: 10.7554/eLife.74314

DNA extraction and 16S amplicon sequencing

Authors: Steven L. Taylor and Geraint B. Rogers
Date: Sep 18, 2022，Views：557

Original protocol used in research article：

Ear microbiota and middle ear disease: a longitudinal pilot study of Aboriginal children in a remote south Australian setting
BMC Microbiology, Jan 13, 2022; DOI: 10.1186/s12866-022-02436-x

TcGDT1-3xHA overexpression

Authors: Srinivasan Ramakrishnan and Roberto Docampo
Date: Sep 17, 2022，Views：563

Original protocol used in research article：

Deletion of a Golgi protein in Trypanosoma cruzi reveals a critical role for Mn2+ in protein glycosylation needed for host cell invasion and intracellular replication
PLoS Pathogens, Mar 15, 2021; DOI: 10.1371/journal.ppat.1009399

Glucose (2-NBDG) uptake assay

Authors: Arpankumar Choksi and Samit Chattopadhyay
Date: Sep 17, 2022，Views：1190

Original protocol used in research article：

Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
Cancer & Metabolism, Apr 16, 2021; DOI: 10.1186/s40170-021-00252-x

Sporulation Test

Authors: Victor Chinomso Ujor, Venkat Gopalan and Thaddeus Chukwuemeka Ezeji
Date: Sep 16, 2022，Views：604

Original protocol used in research article：

Ribozyme-Mediated Downregulation Uncovers DNA Integrity Scanning Protein A (DisA) as a Solventogenesis Determinant in Clostridium beijerinckii
Frontiers in Bioengineering and Biotechnology, Jun 8, 2021; DOI: 10.3389/fbioe.2021.669462

Plaque Reduction Neutralization Test (PRNT)

Authors: Sai Luo, Tom Kirchhausen, Bing Chen, Barton F. Haynes and Frederick W. Alt
Date: Sep 16, 2022，Views：610

Original protocol used in research article：

An Antibody from Single Human VH-rearranging Mouse Neutralizes All SARS-CoV-2 Variants Through BA.5 by Inhibiting Membrane Fusion
Science Immunology, Aug 11, 2022; DOI: 10.1126/sciimmunol.add5446

LX-2 cell culture

Author: Scott L. Friedman
Date: Sep 16, 2022，Views：1001

Original protocol used in research article：

Aramchol downregulates stearoyl CoA-desaturase 1 in hepatic stellate cells to attenuate cellular fibrogenesis
JHEP Reports, Jan 28, 2021; DOI: 10.1016/j.jhepr.2021.100237

Spatially particle-based simulation of FAKY397 activation in integrin clusters

Authors: Bo Cheng and Min Lin
Date: Sep 16, 2022，Views：538

Original protocol used in research article：

Nanoscale integrin cluster dynamics controls cellular mechanosensing via FAKY397 phosphorylation
Science Advances, Mar 4, 2020; DOI: 10.1126/sciadv.aax1909

Immunoblot

Authors: Ziru Li and Ormond A MacDougald
Date: Sep 14, 2022，Views：627

Original protocol used in research article：

Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496

RNA extraction and quantitative real-time PCR (qPCR)

Authors: Ziru Li and Ormond A MacDougald
Date: Sep 14, 2022，Views：638

Original protocol used in research article：

Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496

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