Protocols shared by authors via RaP service
Journal
Observation and motility assay of zebrafish spermatozoa
Authors: Masahide Kikkawa and Hiroshi Yamaguchi
Date: Nov 18, 2019，Views：1250
Original protocol used in research article：
Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly
eLife, May 9, 2018; DOI: 10.7554/eLife.36979
Western blots
Author: Joel Hrit
Date: Nov 18, 2019，Views：1107
Original protocol used in research article：
OGT binds a conserved C-terminal domain of TET1 to regulate TET1 activity and function in development
eLife, Oct 16, 2018; DOI: 10.7554/eLife.34870
4TU metabolic labeling and RNA analysis
Author: Leon Chan
Date: Nov 18, 2019，Views：1495
Original protocol used in research article：
Non-invasive measurement of mRNA decay reveals translation initiation as the major determinant of mRNA stability
eLife, Sep 7, 2018; DOI: 10.7554/eLife.32536
Immunofluorescence assay for 5hmc
Author: Vladimir Camarena
Date: Nov 18, 2019，Views：1406
Original protocol used in research article：
cAMP signaling regulates DNA hydroxymethylation by augmenting the intracellular labile ferrous iron pool
eLife, Dec 14, 2017; DOI: 10.7554/eLife.29750
GFP dropout screening
Author: Jason Sheltzer
Date: Nov 18, 2019，Views：1428
Original protocol used in research article：
CRISPR/Cas9 mutagenesis invalidates a putative cancer dependency targeted in on-going clinical trials
eLife, Mar 24, 2017; DOI: 10.7554/eLife.24179
Rotarod (Ugo Basile Biological Research Apparatus)
Author: Cristian A Lasagna-Reeves
Date: Nov 18, 2019，Views：2000
Original protocol used in research article：
Ataxin-1 oligomers induce local spread of pathology and decreasing them by passive immunization slows Spinocerebellar ataxia type 1 phenotypes
eLife, Dec 17, 2015; DOI: 10.7554/eLife.10891
Detailed protocol for mouse adipose tissue protein extraction
Authors: Yu An and Philipp E Scherer
Date: Nov 18, 2019，Views：4622
Original protocol used in research article：
Angiopoietin-2 in white adipose tissue improves metabolic homeostasis through enhanced angiogenesis
eLife, Mar 29, 2017; DOI: 10.7554/eLife.24071
Native-PAGE to study BiP oligomeric complexes
Author: Steffen Preissler
Date: Nov 18, 2019，Views：6834
Original protocol used in research article：
AMPylation matches BiP activity to client protein load in the endoplasmic reticulum
eLife, Dec 17, 2015; DOI: 10.7554/eLife.12621
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