微生物学

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    现刊
    Sulfatase Assay to Determine Influence of Plants on Microbial Activity in Soil
    硫酸酯酶法测定植物对土壤微生物活性的影响
    作者:Anna Koprivova, Achim Schmalenberger and Stanislav Kopriva日期:01/20/2020,浏览量:1067,Q&A: 0
    [Abstract] Sulfatase activity is often used as a measure of the activity of soil microorganisms. It is thus a suitable tool to investigate the response of microbes to plants. Here we present a method to determine the influence of various Arabidopsis genotypes on the function of soil microbiota using the sulfatase as a quantitative measure. We grew ...
    Steady-state and Flux-based Trehalose Estimation as an Indicator of Carbon Flow from Gluconeogenesis or Glycolysis
    以基于稳态和通量的海藻糖测定作为糖异生或糖酵解碳流指标
    作者:Ritu Gupta and Sunil Laxman日期:01/05/2020,浏览量:1232,Q&A: 0
    [Abstract] Trehalose (and glycogen) is a major storage carbohydrate in many cells, including S. cerevisiae. Typically, trehalose (a disaccharide of glucose) is synthesized and stored through gluconeogenesis. However, trehalose can also be made directly from glucose, if glucose-6-phosphate is channeled away from glycolysis or pentose phosphate ...
    I Plate-based Assay for Studying How Fungal Volatile Compounds (VCs) Affect Plant Growth and Development and the Identification of VCs via SPME-GC-MS
    用于研究真菌挥发性化合物(VCs)如何影响植物生长发育并通过SPME-GC-MS技术鉴定VCs的I板检测法
    作者:Wenzhao Wang, Ningxiao Li, Xingzhong Liu and Seogchan Kang日期:02/20/2019,浏览量:2907,Q&A: 0
    [Abstract] Biogenic volatile compounds (VCs) mediate various types of crucial intra- and inter-species interactions in plants, animals, and microorganisms owing to their ability to travel through air, liquid, and porous soils. To study how VCs produced by Verticillium dahliae, a soilborne fungal pathogen, affect plant growth and development, we ...
    Soluble and Solid Iron Reduction Assays with Desulfitobacterium hafniense
    哈夫尼脱亚硫酸杆菌用于可溶和固体铁的还原试验
    作者:Lucrezia Comensoli, Julien Maillard, Wafa M. Kooli, Pilar Junier and Edith Joseph日期:09/05/2018,浏览量:2449,Q&A: 0
    [Abstract] There is a pressing need to develop sustainable and efficient methods to protect and stabilize iron objects. To develop a conservation-restoration method for corroded iron objects, this bio-protocol presents the steps to investigate reductive dissolution of ferric iron and biogenic production of stabilizing ferrous iron minerals in the strict ...
    Enzymatic Activity Assay for Invertase in Synechocystis Cells
    集胞藻细胞中转化酶酶活的测定方法
    作者:Xiaoming Tan, Kuo Song and Xuefeng Lu日期:05/20/2018,浏览量:3674,Q&A: 0
    [Abstract] Invertase can catalyze the hydrolysis of sucrose, and is widely distributed in cells of cyanobacteria and plants. Being responsible for the first step for sucrose metabolism, invertase plays important physiological roles and its enzymatic activity is frequently needed to be determined. All the methods for determination of the invertase activity ...
    Quantification of Hydrogen Sulfide and Cysteine Excreted by Bacterial Cells
    定量测定细菌细胞分泌的硫化氢和半胱氨酸
    作者:Sergey Korshunov and James A. Imlay日期:05/20/2018,浏览量:3478,Q&A: 0
    [Abstract] Bacteria release cysteine to moderate the size of their intracellular pools. They can also evolve hydrogen sulfide, either through dissimilatory reduction of oxidized forms of sulfur or through the deliberate or inadvertent degradation of intracellular cysteine. These processes can have important consequences upon microbial communities, because ...
    Determination of Intracellular Osmolytes in Cyanobacterial Cells
    蓝藻细胞内渗透调节物质的测定
    作者:Xiaoming Tan, Kuo Song, Cuncun Qiao and Xuefeng Lu日期:04/20/2018,浏览量:3029,Q&A: 0
    [Abstract] Most of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol was applicable to at least four kinds of ...
    Flow Cytometric Quantification of Fatty Acid Uptake by Mycobacterium tuberculosis in Macrophages
    流式细胞术定量测定巨噬细胞中结核分枝杆菌对脂肪酸的摄取
    作者:Evgeniya V. Nazarova, Maria Podinovskaia, David G. Russell and Brian C. VanderVen日期:02/20/2018,浏览量:6338,Q&A: 0
    [Abstract] Mycobacterium tuberculosis (Mtb) has evolved to assimilate fatty acids from its host. However, until recently, there was no reliable way to quantify fatty acid uptake by the bacteria during host cell infection. Here we describe a new method to quantify fatty acid uptake by intracellular bacilli. We infect macrophages with Mtb ...
    Determination of Mn Concentrations in Synechocystis sp. PCC6803 Using ICP-MS
    采用ICP-MS测定集胞藻PCC6803中Mn的浓度
    作者:Fabian Brandenburg, Hanan Schoffman, Nir Keren and Marion Eisenhut日期:12/05/2017,浏览量:4299,Q&A: 0
    [Abstract] Manganese (Mn) is an essential micronutrient for all photoautotrophic organisms. Two distinct pools of Mn have been identified in the cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis), with 80% of the Mn residing in the periplasm and 20% in cytoplasm and thylakoid lumen (Keren et al., 2002). In this protocol, we ...
    Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
    流式细胞术定量分析细菌聚羟基丁酸酯含量
    作者:Antonio Lagares (Jr.) and Claudio Valverde日期:12/05/2017,浏览量:4183,Q&A: 0
    [Abstract] We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously reported works.