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关键词: 水稻, 叶片, 失水速率


  1. 塑料袋
  2. 冰盒
  3. 干净滤纸


  1. 分析天平
  2. 剪刀


  1. 在田间或温室用剪刀迅速剪取水稻完全展开叶片3-6片,至塑料袋中,再放置于冰盒中。
  2. 迅速带回实验室,将叶片取出摊开放置于干净滤纸上,每一样品设3个重复,按一定次序开始用分析天平称量并计数。
  3. 第一次称量记为0时刻,以后每半小时测一次鲜重,直至鲜重不再发生明显改变(约需6-8小时,失水率为60-70%)。
  4. 0时刻鲜重减去各时间点鲜重即为各时间点失水量,各时间点失水量再除以0时刻鲜重即为各时间点失水速率。
  5. 以时间点为横坐标,失水速率为纵坐标绘失水速率图,对每一时间点失水速率进行统计分析,判断两样品间失水速率是否有差异。


  1. 取样时试验样品和对照要相同时期,相同位置,大小相近。
  2. 从冰盒中取出后,将叶表面水分擦干后再称量。
  3. 若将叶片剪短称量,则片段大小应该大小相近,位置相同。
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Copyright: © 2018 The Authors; exclusive licensee Bio-protocol LLC.
引用格式:都浩, 游均, 熊立仲. (2018). 水稻离体叶片失水速率测定. Bio-101: e1010159. DOI: 10.21769/BioProtoc.1010159.
How to cite: Du, H., You, Y. and Xiong, L. Z. (2018). Determination of Water Loss Rate of Excised-rice Leaves. Bio-101: e1010159. DOI: 10.21769/BioProtoc.1010159.

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