Plant Science

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    Measurement of Ascorbate Peroxidase Activity in Sorghum
    Author:  Praveen Kumar, date: 10/20/2022, view: 722, Q&A: 0

    The ascorbate peroxidase (APX) is a widely distributed antioxidant enzyme. It differs from catalase and other peroxidases in that it scavenges/reduces reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) to water using reduced ascorbate as

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    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    Authors:  Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki Koyama and Sanjib Kumar Panda, date: 06/20/2022, view: 1618, Q&A: 0

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial

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    Pneumatic Method to Measure Plant Xylem Embolism
    Authors:  Paulo R. L. Bittencourt, Luciano Pereira and Rafael S. Oliveira, date: 10/20/2018, view: 5513, Q&A: 0
    Embolism, the formation of air bubbles in the plant water transport system, has a major impact on plant water relations. Embolism formation in the water transport system of plants disrupts plant water transport capacity, impairing plant functioning ...
    Protocol to Test the Effect of Sorbitol in vitro on Hybrid Larch (Larix x eurolepis Henry) Emblings
    Authors:  Bettina Johst and María del Carmen Dacasa Rüdinger, date: 07/20/2018, view: 4219, Q&A: 0
    This protocol presents a method to test the effect of sorbitol in vitro on hybrid larch plants derived from somatic embryogenesis. We have tested four different media variants, one control variant without sorbitol and three variants of ...
    Increasing the Membrane Permeability of a Fern with DMSO
    Author:  Marcelo Garcés, date: 06/20/2018, view: 4229, Q&A: 0
    Cell membrane prevents the entrance of extra molecules (e.g., transcription and translation inhibitors) into the cell. For studying the physiological effects of transcription and translation inhibitors on Hymenophyllum caudiculatum ...
    Slow and Fast Desiccation of Single-cell Thick Fronds of Filmy Ferns
    Author:  Marcelo Garcés, date: 06/05/2018, view: 4405, Q&A: 0
    Filmy ferns can desiccate and recover after rehydration to resume photosynthesis. Slow and fast desiccation rates were compared in filmy fern fronds to determine whether structural or physiological differences may occur between desiccation rates. ...
    Measurement of Arabidopsis thaliana Plant Traits Using the PHENOPSIS Phenotyping Platform
    High-throughput phenotyping of plant traits is a powerful tool to further our understanding of plant growth and its underlying physiological, molecular, and genetic determinisms. This protocol describes the methodology of a standard phenotyping ...
    Monitoring Xylem Hydraulic Pressure in Woody Plants
    Xylem sap circulates under either positive or negative hydraulic pressure in plants. Negative hydraulic pressure (i.e., tension) is the most common situation when transpiration is high, and several devices have been developed to quantify it ...
    Assessing Plant Tolerance to Acute Heat Stress
    Authors:  Minsoo Kim, Fionn McLoughlin, Eman Basha and Elizabeth Vierling, date: 07/20/2017, view: 10911, Q&A: 1
    It is well-established that plants are able to acclimate to temperatures above or below the optimal temperature for their growth. Here, we provide protocols for assays that can be used quantitatively or qualitatively to assess the relative ability ...
    Forest GPP Calculation Using Sap Flow and Water Use Efficiency Measurements
    Authors:  Fyodor Tatarinov, Eyal Rotenberg, Dan Yakir and Tamir Klein, date: 04/20/2017, view: 7961, Q&A: 0
    This is a protocol to evaluate gross primary productivity (GPP) of a forest stand based on the measurements of tree’s sap flow (SF), 13C derived water use efficiency (WUE), and meteorological (met) data. GPP was calculated from WUE and ...



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