Plant Science


    Maize Seedlings Colonization with Serendipita indica and Its Colonization Efficiency Analysis
    Authors:  Om Prakash Narayan, Bindu Yadav, Nidhi Verma, Meenakshi Dua and Atul Kumar Johri, date: 10/20/2023, view: 278, Q&A: 0

    Maize is one of the most important crops in the world, and ensuring its successful growth and productivity is crucial for global food security. One way to enhance maize growth and productivity is by improving the colonization of its roots by

    A Plate Growth Assay to Quantify Embryonic Root Development of Zea mays
    Authors:  Jason T. Roberts, Tyler J. McCubbin and David M. Braun, date: 10/20/2023, view: 407, Q&A: 0

    Murashige-Skoog medium solutions have been used in a variety of plant plate growth assays, yet most research uses Arabidopsis thaliana as the study organism. For larger seeds such as maize (Zea mays), most protocols employ a paper towel roll method

    Botrytis cinerea in vivo Inoculation Assays for Early-, Middle- and Late-stage Strawberries
    Authors:  Piao Yang, Zhenzhen Zhao, Aleksandra Virag, Todd Becker, Lijing Zhao, Wenshan Liu and Ye Xia, date: 10/20/2023, view: 277, Q&A: 0

    Strawberries are delicious and nutritious fruits that are widely cultivated and consumed around the world, either fresh or in various products such as jam, juice, and ice cream. Botrytis cinerea is a fungal pathogen that causes gray mold disease on

    13CO2-labelling and Sampling in Algae for Flux Analysis of Photosynthetic and Central Carbon Metabolism
    Authors:  Or Geffen, David Achaintre and Haim Treves, date: 09/05/2023, view: 250, Q&A: 0

    The flux in photosynthesis can be studied by performing 13CO2 pulse labelling and analysing the temporal labelling kinetics of metabolic intermediates using gas or liquid chromatography linked to mass spectrometry. Metabolic flux analysis (MFA) is

    Quantification of Botrytis cinerea Growth in Arabidopsis thaliana
    Authors:  Patricia Scholz, Kent D. Chapman, Till Ischebeck and Athanas Guzha, date: 08/20/2023, view: 548, Q&A: 0

    Yield losses attributed to plant pathogens pose a serious threat to plant productivity and food security. Botrytis cinerea is one of the most devastating plant pathogens, infecting a wide array of plant species; it has also been established as a

    A Simple Sonication Method to Isolate the Chloroplast Lumen in Arabidopsis thaliana
    Authors:  Jingfang Hao and Alizée Malnoë, date: 08/05/2023, view: 364, Q&A: 0

    The chloroplast lumen contains at least 80 proteins whose function and regulation are not yet fully understood. Isolating the chloroplast lumen enables the characterization of the lumenal proteins. The lumen can be isolated in several ways through

    Bi-directional Dual-flow-RootChip for Physiological Analysis of Plant Primary Roots Under Asymmetric Perfusion of Stress Treatments
    Authors:  Claudia Allan, Blake Elliot, Volker Nock and Claudia-Nicole Meisrimler, date: 08/05/2023, view: 316, Q&A: 0

    Due to technical limitations, research to date has mainly focused on the role of abiotic and biotic stress–signalling molecules in the aerial organs of plants, including the whole shoot, stem, and leaves. Novel experimental platforms including

    A Novel Method for Measuring Mitochondrial Respiratory Parameters in Wheat Paleae (Paleae Superior) Using the XF24 Analyzer
    Authors:  Daniel Schniertshauer and Jörg Bergemann, date: 08/05/2023, view: 148, Q&A: 0

    Understanding the influence of secondary metabolites from fungi on the mitochondria of the host plant during infection is of great importance for the knowledge of fungus–plant interactions in general; it could help generate resistant plants in

    An Improved System to Measure Leaf Gas Exchange on Adaxial and Abaxial Surfaces
    Authors:  D. A. Márquez, H. Stuart-Williams, S. C. Wong and G. D. Farquhar, date: 06/05/2023, view: 300, Q&A: 0

    Measurement of leaf carbon gain and water loss (gas exchange) in planta is a standard procedure in plant science research for attempting to understand physiological traits related to water use and photosynthesis. Leaves carry out gas exchange

    Determination of Paraquat in Arabidopsis Tissues and Protoplasts by UHPLC-MS/MS
    Authors:  Mingming Zhao, Qi Wang, Muyu Shi, Ziyan Sun, Huiru Tang and Xiaochun Ge, date: 04/05/2023, view: 422, Q&A: 0

    Paraquat is a cost-effective herbicide, widely used in many countries, that can induce severe oxidative stress in photosynthetic tissues. Studying plant herbicide resistance or antioxidant stress mechanisms requires determining the cellular paraquat


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