水稻叶片相对含水量测定   

How to cite Favorites Q&A Share your feedback Cited by

Original research article

A brief version of this protocol appeared in:
Rice Protocol e-book

实验原理:水稻叶片在非生物逆境胁迫条件下,叶片含水量有着较大的差异。
实验目的:通过叶片相对含水量的不同,检测水稻在非生物逆境下保持水分的能力,相对含水量越高,则水稻抗失水能力越强。

关键词: 水稻, 叶片, 相对含水量

材料与试剂

  1. 冰盒
  2. 塑料袋
  3. 干净滤纸
  4. 50 ml离心管

仪器设备

  1. 干燥箱
  2. 分析天平
  3. 剪刀

实验步骤

  1. 在田间或温室用剪刀迅速剪取水稻完全展开叶片3-6片,至塑料袋中,再放置于冰盒中。
  2. 迅速带回实验室,将叶片取出摊开放置于干净滤纸上,按一定次序开始用分析天平称量鲜重记录为m1
  3. 将叶片放到50 ml离心管中,自来水浸泡2 h后将叶表面水分擦干,迅速称量记录m2
  4. 将叶片置于60 °C干燥箱10~15 h,至无水分丧失。称量干重记录m3
  5. 计算公式:
    叶片相对含水量(%) = (m1-m3)/(m2-m3)×100%
    叶片含水量(%) = (m1-m3)/m1×100%

注意事项

  1. 取样时试验样品和对照要相同时期,相同位置,大小相近。
  2. 从冰盒中取出后,将叶表面水分擦干后再称量。
  3. 若将叶片剪短称量,则片段大小应该大小相近,位置相同。
Copyright: © 2018 The Authors; exclusive licensee Bio-protocol LLC.
引用格式:都浩, 郭子龙, 熊立仲. (2018). 水稻叶片相对含水量测定. Bio-101: e1010157. DOI: 10.21769/BioProtoc.1010157.
How to cite: Du, H., Guo, Z. L. and Xiong, L. Z. (2018). Determination of Relative Rice Leaf Water Content. Bio-101: e1010157. DOI: 10.21769/BioProtoc.1010157.
Q&A

Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.

You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.