实验原理:用石蜡包埋组织块制作的显微切片。经取材、固定、洗涤和脱水、透明、浸蜡、包埋、切片与粘片、脱蜡、染色、脱水、透明、封片等步骤。在显微镜下可清晰观察细胞组织的形态结构。 实验目的:石蜡切片是研究细胞生物学的基础技术,不仅是研究组织细胞形态的重要手段,更是RNA原位杂交、TUNEL法检测细胞凋亡、免疫组织化学等分子细胞生物学的基本步骤。
关键词: 固定, 包埋, 组织切片
材料与试剂
仪器设备
实验步骤
注意事项
溶液配方
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