Cell Biology


    An Improved Protocol for the Matrigel Duplex Assay: A Method to Measure Retinal Angiogenesis

    Neovascular diseases of the retina, such as diabetic retinopathy (DR) and age-related macular degeneration (AMD), are proliferative retinopathies involving the growth of new blood vessels on the retina, which in turn causes impairment and potential

    Princeton RAtlas: A Common Coordinate Framework for Fully cleared, Whole Rattus norvegicus Brains

    Whole-brain clearing and imaging methods are becoming more common in mice but have yet to become standard in rats, at least partially due to inadequate clearing from most available protocols. Here, we build on recent mouse-tissue clearing and

    Recoil Measurements in Drosophila Embryos: from Mounting to Image Analysis
    Authors:  Luis Eduardo Sánchez-Cisneros, Sourabh Bhide and Luis Daniel Ríos-Barrera, date: 07/20/2023, view: 354, Q&A: 0

    Tension and force propagation play a central role in tissue morphogenesis, as they enable sub- and supra-cellular shape changes required for the generation of new structures. Force is often generated by the cytoskeleton, which forms complex

    Systematic Analysis of Smooth Muscle and Cartilage Ring Formation during Mouse Tracheal Tubulogenesis
    Authors:  Haoyu Wu, Ping Wang, Ziying Liu, Chunyan Lu and Wenguang Yin, date: 07/05/2023, view: 186, Q&A: 0

    The trachea tube is the exclusive route to allow gas exchange between the external environment and the lungs. Recent studies have shown the critical role of mesenchymal cells in tracheal tubulogenesis. Improved methods for studying the dynamics of

    In vivo Electroporation of Skeletal Muscle Fibers in Mice
    Authors:  Steven J. Foltz, H. Criss Hartzell and Hyojung J. Choo, date: 07/05/2023, view: 298, Q&A: 0

    In vitro models are essential for investigating the molecular, biochemical, and cell-biological aspects of skeletal muscle. Still, models that utilize cell lines or embryonic cells do not fully recapitulate mature muscle fibers in vivo. Protein

    THRIFTY—A High-throughput Single Muscle Fiber Typing Method Based on Immunofluorescence Detection
    Authors:  Sebastian Edman, Oscar Horwath and William Apró, date: 05/20/2023, view: 394, Q&A: 0

    Skeletal muscle consists of a mixture of fiber types with different functional and metabolic characteristics. The relative composition of these muscle fiber types has implications for muscle performance, whole-body metabolism, and health. However,

    E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
    Authors:  Elena Astanina, Sara Petrillo, Tullio Genova, Federico Mussano and Federico Bussolino, date: 05/05/2023, view: 306, Q&A: 0

    X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology

    Induction of Skeletal Muscle Injury by Intramuscular Injection of Cardiotoxin in Mouse
    Authors:  Xin Fu, Sheng Li, Minzhi Jia, Wenjun Yang and Ping Hu, date: 05/05/2023, view: 850, Q&A: 0

    Skeletal muscle is the most abundant tissue in the human body and has a tremendous capability to regenerate in response to muscle injuries and diseases. Induction of acute muscle injury is a common method to study muscle regeneration in vivo.

    In situ Microinflammation Detection Using Gold Nanoclusters and a Tissue-clearing Method

    Microinflammation enhances the permeability of specific blood vessel sites through an elevation of local inflammatory mediators, such as interleukin (IL)-6 and tumor necrosis factor (TNF)-α. By a two-dimensional immunohistochemistry analysis

    Live Imaging of Phagoptosis in ex vivo Drosophila Testis
    Authors:  Diana Kanaan, Boris Shklyar, Lilach Porat-Kuperstein and Hila Toledano, date: 03/20/2023, view: 577, Q&A: 0

    Phagoptosis is a prevalent type of programmed cell death (PCD) in adult tissues in which phagocytes non-autonomously eliminate viable cells. Therefore, phagoptosis can only be studied in the context of the entire tissue that includes both the ...

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