Cell Biology


    Princeton RAtlas: A Common Coordinate Framework for Fully cleared, Whole Rattus norvegicus Brains

    Whole-brain clearing and imaging methods are becoming more common in mice but have yet to become standard in rats, at least partially due to inadequate clearing from most available protocols. Here, we build on recent mouse-tissue clearing and

    Recoil Measurements in Drosophila Embryos: from Mounting to Image Analysis
    Authors:  Luis Eduardo Sánchez-Cisneros, Sourabh Bhide and Luis Daniel Ríos-Barrera, date: 07/20/2023, view: 354, Q&A: 0

    Tension and force propagation play a central role in tissue morphogenesis, as they enable sub- and supra-cellular shape changes required for the generation of new structures. Force is often generated by the cytoskeleton, which forms complex

    THRIFTY—A High-throughput Single Muscle Fiber Typing Method Based on Immunofluorescence Detection
    Authors:  Sebastian Edman, Oscar Horwath and William Apró, date: 05/20/2023, view: 394, Q&A: 0

    Skeletal muscle consists of a mixture of fiber types with different functional and metabolic characteristics. The relative composition of these muscle fiber types has implications for muscle performance, whole-body metabolism, and health. However,

    E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT
    Authors:  Elena Astanina, Sara Petrillo, Tullio Genova, Federico Mussano and Federico Bussolino, date: 05/05/2023, view: 306, Q&A: 0

    X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology

    In situ Microinflammation Detection Using Gold Nanoclusters and a Tissue-clearing Method

    Microinflammation enhances the permeability of specific blood vessel sites through an elevation of local inflammatory mediators, such as interleukin (IL)-6 and tumor necrosis factor (TNF)-α. By a two-dimensional immunohistochemistry analysis

    Live Imaging of Phagoptosis in ex vivo Drosophila Testis
    Authors:  Diana Kanaan, Boris Shklyar, Lilach Porat-Kuperstein and Hila Toledano, date: 03/20/2023, view: 577, Q&A: 0

    Phagoptosis is a prevalent type of programmed cell death (PCD) in adult tissues in which phagocytes non-autonomously eliminate viable cells. Therefore, phagoptosis can only be studied in the context of the entire tissue that includes both the ...

    Maximizing the Rod Outer Segment Yield in Retinas Extracted from Cattle Eyes
    Authors:  Isabella Panfoli, Daniela Calzia, Silvia Ravera, Paolo Bianchini and Alberto Diaspro, date: 07/20/2022, view: 1019, Q&A: 0

    The retina is a thin neuronal multilayer responsible for the detection of visual information. The first step in visual transduction occurs in the photoreceptor outer segment. The studies on photoreception and visual biochemistry have often utilized

    Whole-mount Senescence-Associated Beta-Galactosidase (SA-β-GAL) Activity Detection Protocol for Adult Zebrafish
    Authors:  Marta Marzullo, Mounir El Maï and Miguel Godinho Ferreira, date: 07/05/2022, view: 2727, Q&A: 0

    Senescence-associated beta-galactosidase (SA-β-GAL) is an enzyme that accumulates in the lysosomes of senescent cells, where it hydrolyses β-galactosides. With p16, it represents a well-recognized biomarker used to assess senescence both

    Preparation of a Single Cell Suspension from the Murine Iridocorneal Angle
    Authors:  Benjamin R. Thomson and Susan E. Quaggin, date: 05/20/2022, view: 1355, Q&A: 0

    Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified

    Ex vivo Human Skin Infection with Herpes Simplex Virus 1
    Authors:  Nydia C. De La Cruz, Maureen Möckel, Lisa Wirtz and Dagmar Knebel-Mörsdorf, date: 05/05/2022, view: 1182, Q&A: 0

    Although herpes simplex virus 1 (HSV-1) is a well-studied virus, how the virus invades its human host via skin and mucosa to reach its receptors and initiate infection remains an open question. For studies of HSV-1 infection in skin, mice have been


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