微生物学

分类

    现刊
    Construction of a Highly Diverse mRNA Library for in vitro Selection of Monobodies
    体外选择单体的高度多样性mRNA文库的构建
    [Abstract]

    Recently, we developed transcription/translation coupled with the association of puromycin linker (TRAP) display as a quick in vitro selection method to obtain antibody-like proteins. For the in vitro selection, it is important to prepare mRNA libraries among which the diversity is high. Here, we describe a method for the preparation of monobody

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    Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
    蛋白质pull-down免疫共沉淀实验分析病毒DNA结合蛋白质之间的直接相互作用
    作者:Ana Lechuga, Mónica Berjón-Otero, Margarita Salas and Modesto Redrejo-Rodríguez日期:01/05/2018,浏览量:8955,Q&A: 0
    [Abstract] This protocol analyzes the direct interaction between two DNA-binding proteins by pull-down co-immunoprecipitation. One of the proteins is overexpressed in E. coli as HA-tagged recombinant protein and cell-free extracts are immunoprecipitated in HA-affinity resin. Cell extracts are treated with nuclease to degrade DNA and RNA, which rules ...
    Chase Assay of Protein Stability in Haloferax volcanii
    沃氏嗜盐富饶菌中蛋白质稳定性的追踪测定
    作者:Xian Fu and Julie A. Maupin-Furlow日期:03/20/2017,浏览量:6303,Q&A: 0
    [Abstract] Highly regulated and targeted protein degradation plays a fundamental role in almost all cellular processes. Determination of the protein half-life by the chase assay serves as a powerful and popular strategy to compare the protein stability and study proteolysis pathways in cells. Here, we describe a chase assay in Haloferax volcanii, a ...
    Coupling of HIV-1 gp120-derived Core Protein to Paramagnetic Beads and Adsorption Assays
    基于磁珠分选及固相吸附分析针对HIV-1 gp120核心蛋白的抗体
    作者:Jidnyasa Ingale and Richard T Wyatt日期:10/05/2015,浏览量:6581,Q&A: 0
    [Abstract] Analysis of the functional activity in polyclonal serum following immunization of a complex protein or glycoprotein immunogen is a very important but tedious process. Fine mapping of epitope-specific antibodies is difficult when they are elicited at relatively low levels. In our recent study focused on developing an HIV-1 vaccine, we immunized ...
    Preparation of Parasite Protein Extracts and Western Blot Analysis
    寄生虫蛋白质提取物的制备和蛋白印迹分析
    作者:Arlett Heiber and Tobias Spielmann日期:06/05/2014,浏览量:23232,Q&A: 0
    [Abstract] In order to prepare protein extracts of Plasmodium falciparum blood stages for western blot analysis, infected red blood cells (iRBC) need to be separated from uninfected red blood cells (uRBC) which make up the bulk of the parasite culture. Depending on the localisation of the parasite protein of interest, different methods are available ...
    Western Blotting for Staphylococcus aureus AgrA
    葡萄球菌属AgrA蛋白印迹实验
    作者:Chikara Kaito and Kazuhisa Sekimizu日期:03/20/2014,浏览量:12145,Q&A: 0
    [Abstract] Staphylococcus aureus has a quorum sensing system to regulate the expression of various virulence factors, which is exerted by the agr locus that encodes agrBDCA and a regulatory RNA called RNAIII. AgrB, AgrD, and AgrC proteins are involved in producing and recognizing extracellular quorum sensing molecules and transduce ...
    Co-immunoprecipitation of Flag-TLR3 or Myc-MSR1 with HCV RNA
    Flag-TLR3 或 Myc-MSR1 与 HCV RNA的免疫共沉淀
    作者:Daisuke Yamane, Hiromichi Dansako and Stanley M. Lemon日期:03/05/2014,浏览量:9449,Q&A: 0
    [Abstract] Co-immunoprecipitation assay of TLR3-Flag or Myc-MSR1 with HCV RNA is used to identify direct interaction of viral RNA with host proteins that recognize viral RNA to initiate interferon (IFN) signaling, a crucial antiviral response of the host cells. Both Toll-like receptor 3 (TLR3) and class-A scavenger receptor type 1 (MSR1) proteins recognize ...
    Cyclic Nucleotide (cAMP and cGMP) Assays and Capture ELISA for Quantitative Analysis of Plasmodium falciparum Blood-stage Egress
    采用环核苷酸(cAMP和cGMP)试验和捕获酶联免疫吸附法量化分析恶性疟原虫红内期外出
    作者:Fiona Hackett, Christine R Collins, Malcolm Strath and Michael J Blackman日期:03/05/2014,浏览量:10121,Q&A: 0
    [Abstract] Upon rupture of Plasmodium falciparum (P. falciparum) schizonts in vitro (an event known as egress), merozoites are released into the culture medium. The merozoites invade fresh red blood cells, a process that involves shedding of a microneme protein called apical membrane antigen-1 (AMA1) from the merozoite surface. ...
    Shigella IpaD and IpaB Surface Localizations
    志贺氏杆菌IPaD和IPAB表面定位
    作者:Lionel Schiavolin, Alaeddine Meghraoui and Abdelmounnaim Allaoui日期:11/20/2013,浏览量:10147,Q&A: 1
    [Abstract] Shigella uses a type III secretion system to invade host cell and to cause disease. Secretion control and insertion of a translocation pore into cell membrane are critical steps for pathogenesis and are tightly linked to the formation of the needle tip complex formed by the IpaB and IpaD proteins (Veenendaal et al., 2007). ...
    Immunoblot Analysis of Histone H4 Acetylation and Histone H2A Phosphorylation in Candida albicans
    白色念珠菌中组蛋白H4乙酰化和组蛋白H2A磷酸化的免疫印迹分析
    作者:Michael Tscherner and Karl Kuchler日期:10/20/2013,浏览量:8772,Q&A: 0
    [Abstract] Posttranslational modifications of histones are required for different processes including transcription, replication and DNA damage repair. This protocol describes the preparation of a whole-cell extracts for the fungal pathogen Candida albicans. Furthermore, the extract is used to detect lysine acetylation of histone H4 as well as ...