联合评审(
Aksiniya Asenova University of California, Davis
15 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
51 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

Amit Kumar Dey International Centre for Genetic Engineering and Biotechnology
17 protocols

出版人
Fanglian He
  • Publisher, Bio-protocol
研究方向
  • 微生物学
  • Host-bacterium interaction
  • 已发表 protocol 19

教育背景

Ph.D., University of Pennsylvania, USA

发表论文

已发表 protocol 19篇
Alkaline Phosphatase
载体5‘磷酸基团的碱性磷酸酶消解

作者:Fanglian He日期:07/20/2012,浏览量:37049,Q&A: 3
Alkaline phosphatase removes 5' phosphate groups from vector so that prevents self-ligation of the vector and facilitates the ligation of other DNA fragments into the vector.
Oil Red O Staining of Fixed Worm
固定蠕虫的油红染色

作者:Fanglian He日期:07/05/2012,浏览量:24498,Q&A: 1
Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco.
参审 protocol 63篇
Quantitation of Cytochromes b559, b6, and f, and the Core Component of Photosystem I P700 in Cyanobacterial Cells
量化蓝藻细胞中的细胞色素b559、b6和f以及光和体系的核心成分I P700
作者:Motohide Aoki, Mikio Tsuzuki and Norihiro Sato日期:11/05/2016,浏览量:6620,Q&A: 0
Cytochrome (Cyt) b559, an important and essential core component of photosystem II in the photosynthetic electron transport system, is a heme-bridged heterodimer protein composed of an alpha subunit (PsbE) and a beta ...
查看全文 >
Quantification of Callose Deposition in Plant Leaves
植物叶中胼胝质沉积的定量测定
Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is widespread in higher plants. Callose is involved in multiple aspects of plant growth and development. It is synthetized in plants at the cell plate during cytokinesis, in ...
查看全文 >
主审 protocol 142篇
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
采用流式细胞术分选术分离高纯度原代小鼠II型肺泡上皮细胞
作者:Meenal Sinha and Clifford A. Lowell日期:11/20/2016,浏览量:20545,Q&A: 1
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
查看全文 >
Extraction and Quantification of Polyphosphate in the Budding Yeast Saccharomyces cerevisiae
出芽酿酒酵母中聚磷酸的提取和定量测定
作者:David Canadell, Samuel Bru, Josep Clotet and Joaquín Ariño日期:07/20/2016,浏览量:9878,Q&A: 0
Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond ...
查看全文 >