联合作者(
Ryan Richard Cupo
1 protocol

Lin Guo Biochemistry and Molecular Biology, Thomas Jefferson University, 2019-
1 protocol

Emma Robinson Department of Biochemistry and Molecular Biology, Thomas Jefferson University, 2019-2020
1 protocol

James Shorter
  • Professor, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, USA
研究方向
  • 生物化学
  • 已发表 protocol 2

教育背景

Ph.D., University of London, U.K.

发表论文

已发表 protocol 2篇
Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli
重组Skd3(人ClpB)蛋白和烟草蚀刻病毒(TEV)蛋白酶在大肠杆菌中的表达与纯化

作者:Ryan R. Cupo and James Shorter日期:12/05/2020,浏览量:1841,Q&A: 0

Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the

...
Karyopherin-β2 Inhibits and Reverses Aggregation and Liquid-liquid Phase Separation of the ALS/FTD-Associated Protein FUS
核转运蛋白-β2抑制和ALS / FTD相关蛋白FUS的逆转聚集和液-液相分离

作者:Emma Robinson, James Shorter and Lin Guo日期:08/20/2020,浏览量:2207,Q&A: 0
The study of RNA-binding proteins (RBP) offers insight into the mechanisms of pathologic protein aggregation in neurodegenerative diseases. We developed a protocol for purifying an RBP FUS and a nuclear import receptor (NIR) Kapβ2 and testing the ...