联合作者(
Elena Mercade Department of Biology, Healthcare and the Environment, University of Barcelona
1 protocol

Carmen López-Iglesias Maastricht Multimodal Molecular Imaging Institute M4I , university of maastricht, 2015-2019
1 protocol

Carla Pérez-Cruz Department of Biology Health and Environment, University, Barcelona
1 protocol

Nicolás Baeza Department of Biology Health and Environment, University of Barcelona, Barcelona
1 protocol

Lidia Delgado
  • Lab Manager, Electron Cryomicroscopy Unit, Scientific and Technological Centers University of Barcelona
研究方向
  • -
  • 已发表 protocol 1

教育背景

Ph.D., University of Barcelona, Spain, 2015

发表论文

• Delgado L, López-Iglesias C, Mercadé E. Deciphering the stack, a novel bacterial structure, by (cryo-) transmission electron microscopy and (cryo-) electron tomography. Recent Advances in Pharmaceutical Sciences VII. Edited by Diego Muñoz-Torrero, Montserrat Riu and Carles Feliu. Research Signpost. ISBN: 9788130805733. 2017.
• Delgado L, Martínez G, López-Iglesias C, Mercadé E. Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack. J Struct Biol. 2015 Mar;189(3):220-9. doi: 10.1016/j.jsb.2015.01.008. Epub 2015 Jan 21. PubMed PMID: 25617813.
• Pérez-Cruz C, Delgado L, López-Iglesias C, Mercadé E. Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. PLoS One. 2015 Jan 12;10(1): e0116896. doi: 10.1371/journal.pone.0116896. eCollection 2015. PubMed PMID: 25581302; PubMed Central PMCID: PMC4291224.
• Carrión O, Delgado L, Mercadé E. New emulsifying and cryoprotective exopolysaccharide from Antarctic Pseudomonas sp. ID1. Carbohydr Polym. 2015 Mar 6;117: 1028-34. doi: 10.1016/j.carbpol.2014.08.060. Epub 2014 Sep 2. PubMed PMID: 25498731.
• Delgado L, Carrión O, Martínez G, López-Iglesias C, Mercadé E. The stack: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1(T) by transmission electron microscopy and tomography. PLoS One. 2013 Sep 9;8(9):e73297. doi: 10.1371/journal.pone.0073297. eCollection 2013. PubMed PMID: 24039905; PubMed Central PMCID: PMC3767748.
• Pérez-Cruz C, Carrión O, Delgado L, Martinez G, López-Iglesias C, Mercade E. New type of outer membrane vesicle produced by the Gram-negative bacterium Shewanella vesiculosa M7T: implications for DNA content. Appl Environ Microbiol. 2013 Mar;79(6):1874-81. doi: 10.1128/AEM.03657-12. Epub 2013 Jan 11. PubMed PMID: 23315742; PubMed Central PMCID: PMC3592255.
• Pujadas L, Gruart A, Bosch C, Delgado L, Teixeira CM, Rossi D, de Lecea L, Martínez A, Delgado-García JM, Soriano E. Reelin regulates postnatal neurogenesis and enhances spine hypertrophy and long-term potentiation. J Neurosci. 2010 Mar 31;30(13):4636-49. doi: 10.1523/JNEUROSCI.5284-09.2010. PubMed PMID: 20357114; PubMed Central PMCID: PMC6632327.
已发表 protocol 1篇
Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria
革兰阴性致病菌自然分泌的外-内膜囊泡低温透射电子显微镜观察

作者:Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias and Elena Mercadé日期:09/20/2019,浏览量:1598,Q&A: 0
A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so ...