联合作者(
Carmen López-Iglesias Maastricht Multimodal Molecular Imaging Institute M4I , university of maastricht, 2015-2019
1 protocol

Carla Pérez-Cruz Department of Biology Health and Environment, University, Barcelona
1 protocol

Nicolás Baeza Department of Biology Health and Environment, University of Barcelona, Barcelona
1 protocol

Lidia Delgado Scientific and Technological Centers University of Barcelona
1 protocol

Elena Mercade
  • Associate professor in Microbiology, Department of Biology, Healthcare and the Environment, University of Barcelona
研究方向
  • 微生物学
  • 已发表 protocol 1

教育背景

PhD in Microbiology, University of Barcelona, Spain, 1990

发表论文

Since 2013
• Arcarons N, Vendrell-Flotats M, Yeste M, Mercadé E, López-Béjar M, Mogas T. Cryoprotectant role of exopolysaccharide of Pseudomonas sp. ID1 in the vitrification of IVM cow oocytes. Reprod Fertil Dev. 2019. May 16. doi: 10.1071/RD18447.
• Curson ARJ, Williams BT, Pinchbeck BJ, Sims LP, Martínez AB, Rivera PPL, Kumaresan D, Mercadé E, Spurgin LG, Carrión O, Moxon S, Cattolico RA, Kuzhiumparambil U, Guagliardo P, Clode PL, Raina JB, Todd JD. DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton. Nat Microbiol. 2018. Apr; 3(4):430-439. doi: 10.1038/s41564-018-0119-5. Erratum in: Nat Microbiol. 2019. Mar;4(3):540-542.
• Magri G, Comerma L, Pybus M, Sintes J, Lligé D, Segura-Garzón D, Bascones S, Yeste A, Grasset EK, Gutzeit C, Uzzan M, Ramanujam M, van Zelm MC, Albero-González R, Vazquez I, Iglesias M, Serrano S, Márquez L, Mercadé E, Mehandru S, Cerutti A. Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals. Immunity. 2017. Jul; 18;47(1):118-134.e8. doi: 10.1016/j.immuni.2017.06.013.
• Delgado L, López-Iglesias C, Mercadé E. Deciphering the stack, a novel bacterial structure, by (cryo-) transmission electron microscopy and (cryo-) electron tomography. Recent Advances in Pharmaceutical Sciences VII. Edited by Diego Muñoz-Torrero, Montserrat Riu and Carles Feliu. Research Signpost. ISBN: 9788130805733. 2017.
• Pérez-Cruz C, Cañas MA, Giménez R, Badia J, Mercadé E, Baldomà L, Aguilera L. Membrane Vesicles Released by a hypervesiculating Escherichia coli Nissle 1917 tolR Mutant Are Highly Heterogeneous and Show Reduced Capacity for Epithelial Cell Interaction and Entry. PLoS One. 2016 Dec; 30;11(12):e0169186. doi: 10.1371/journal.pone.0169186.
• Delgado L, Martínez G, López-Iglesias C, Mercadé E. Cryo-electron tomography of plunge-frozen whole bacteria and vitreous sections to analyze the recently described bacterial cytoplasmic structure, the Stack. J Struct Biol. 2015 Mar; 189(3):220-9. doi: 10.1016/j.jsb.2015.01.008.
• Carrión O, Delgado L, Mercadé E. New emulsifying and cryoprotective exopolysaccharide from Antarctic Pseudomonas sp. ID1. Carbohydr Polym. 2015 Mar 6; 117:1028-34. doi: 10.1016/j.carbpol.2014.08.060.
• Pérez-Cruz C, Delgado L, López-Iglesias C, Mercadé E. Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. PLoS One. 2015.12;10(1):e0116896. doi: 10.1371/journal.pone.0116896.
• Carrión, O.; Curson, A.R.J.; Kumaresan, D.; Yu, Y.; Lang, A.S.; Mercadé, E.; Todd, J.D. A novel pathway producing dimethylsulphide in bacteria is widespread in soil environments. 916151 – Nature Communications. 2015. pp. 1 - 8. doi: 10.1038/ncomms7579
• Pérez-Cruz, C.; Carrión, O.; Delgado, L.; Martinez, G.; López-Iglesias, C.; Mercade, E. A new type of outer membrane vesicles produced by the Gram-negative bacterium Shewanella vesiculosa M7T: implications for DNA content. 905739 - Applied and Environmental Microbiology. 2013. Mar; 79(6):1874-81. doi: 10.1128/AEM.03657-12.
• Delgado,L.; Carrión,O.; Martinez, G.; López-Iglesias, C.; Mercadé, E. The Stacks: a new bacterial structure analyzed in the Antarctic bacterium Pseudomonas deceptionensis M1T by transmission electron microscopy and tomography. 915133 - PLoS One. 2013. Sept; 8(9):e732978 - 9. https://doi.org/10.1371/journal.pone.0073297
已发表 protocol 1篇
Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria
革兰阴性致病菌自然分泌的外-内膜囊泡低温透射电子显微镜观察

作者:Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias and Elena Mercadé日期:09/20/2019,浏览量:1241,Q&A: 0
A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so ...