联合作者(
Marc-David Ruepp Basic and Clinical Neuroscience, King's College London, UK, UK,
1 protocol

Jonas Mechtersheimer Basic and Clinical Neuroscience, King's College London, UK, UK,
1 protocol

Stefan Reber
  • Departement für Chemie und Biochemie, University of Bern, Switzerland, Switzerland,
研究方向
  • 分子生物学
  • 已发表 protocol 1

Education

PhD, University of Bern, Switzerland, 2018

Current position

Research Associate, Department of Basic and Clinical Neuroscience, King’s College London, United Kingdom

Publications

  1. Hock, E. M., Maniecka, Z., Hruska-Plochan, M., Reber, S., Laferriere, F., Sahadevan, M. K. S., Ederle, H., Gittings, L., Pelkmans, L., Dupuis, L., Lashley, T., Ruepp, M. D., Dormann, D. and Polymenidou, M. (2018). Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function. Cell Rep 24(4): 987-1000 e1007.
  2. Reber, S., Mechtersheimer, J., Nasif, S., Benitez, J. A., Colombo, M., Domanski, M., Jutzi, D., Hedlund, E. and Ruepp, M. D. (2018). CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells. Mol Biol Cell 29(2): 75-83.
  3. Reber, S., Stettler, J., Filosa, G., Colombo, M., Jutzi, D., Lenzken, S. C., Schweingruber, C., Bruggmann, R., Bachi, A., Barabino, S. M., Muhlemann, O. and Ruepp, M. D. (2016). Minor intron splicing is regulated by FUS and affected by ALS-associated FUS mutants. EMBO J 35(14): 1504-1521.
  4. Suarez-Calvet, M., Neumann, M., Arzberger, T., Abou-Ajram, C., Funk, E., Hartmann, H., Edbauer, D., Kremmer, E., Gobl, C., Resch, M., Bourgeois, B., Madl, T., Reber, S., Jutzi, D., Ruepp, M. D., Mackenzie, I. R., Ansorge, O., Dormann, D. and Haass, C. (2016). Monomethylated and unmethylated FUS exhibit increased binding to Transportin and distinguish FTLD-FUS from ALS-FUS. Acta Neuropathol 131(4): 587-604.
  5. Raczynska, K. D., Ruepp, M. D., Brzek, A., Reber, S., Romeo, V., Rindlisbacher, B., Heller, M., Szweykowska-Kulinska, Z., Jarmolowski, A. and Schumperli, D. (2015). FUS/TLS contributes to replication-dependent histone gene expression by interaction with U7 snRNPs and histone-specific transcription factors. Nucleic Acids Res 43(20): 9711-9728.
已发表 protocol 1篇
Generation of Gene Knockout and Gene Replacement with Complete Removal of Full-length Endogenous Transcript Using CRISPR-Trap
通过CRISPR-Trap完全移除内源全长转录本进行基因敲除和基因置换

作者:Jonas Mechtersheimer, Stefan Reber and Marc-David Ruepp日期:10/20/2018,浏览量:5667,Q&A: 0
This protocol describes the application of the CRISPR-Trap from designing of the gene targeting strategy to validation of successfully edited clones that was validated on various human cell lines, among them human induced pluripotent stem cells ...