联合作者(
Gregory C. Finnigan Kansas State University
2 protocols

Rachael Marie Giersch
  • Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
  • 已发表 protocol 1
已发表 protocol 1篇
Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
酿酒酵母中使用人工靶DNA序列进行多重CRISPR/ Cas9的方法

作者:Rachael M. Giersch and Gregory C. Finnigan日期:09/20/2017,浏览量:5614,Q&A: 0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...