生物化学

分类

    现刊
    GC-MS-Based Analysis of Methanol: Chloroform-extracted Fatty Acids from Plant Tissues
    基于GC-MS法分析使用甲醇:氯仿从植物组织中提取的脂肪酸
    作者:Manish Kumar Patel, Shubhashis Das and Jitendra Kumar Thakur日期:09/20/2018,浏览量:2971,Q&A: 0
    [Abstract] Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and pharmacological industries due to their nutritional importance. In addition, FAs are considered as a promising ...
    Assessment of Uptake and Biodistribution of Radiolabeled Cholesterol in Mice Using Gavaged Recombinant Triglyceride-rich Lipoprotein Particles (rTRL)
    使用管饲法重组富含甘油三酯的脂蛋白颗粒(rTRL)来评估小鼠中放射性标记的胆固醇的摄取和生物分布
    作者:Anna Worthmann, Clara John and Joerg Heeren日期:07/05/2018,浏览量:1628,Q&A: 0
    [Abstract] The here described method can be used to estimate the uptake of orally provided cholesterol in mice. Briefly, mice are gavaged with radiolabeled cholesterol and 4 h later, organ distribution of the radiolabel is determined by liquid scintillation counting. The method has been applied successfully to determine dietary cholesterol handling of mice ...
    Tracking Lipid Transfer by Fatty Acid Isotopolog Profiling from Host Plants to Arbuscular Mycorrhiza Fungi
    脂肪酸同位素标记谱分析追踪脂质从寄主植物到丛枝菌根真菌的转运
    作者:Andreas Keymer, Claudia Huber, Wolfgang Eisenreich and Caroline Gutjahr日期:04/05/2018,浏览量:2579,Q&A: 0
    [Abstract] Lipid transfer from host plants to arbuscular mycorrhiza fungi was hypothesized for several years because sequenced arbuscular mycorrhiza fungal genomes lack genes encoding cytosolic fatty acid synthase (Wewer et al., 2014; Rich et al., 2017). It was finally shown by two independent experimental approaches (Jiang et al., ...
    Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri
    凯氏小球藻中脂肪酸含量及三酰甘油组成
    作者:Motohide Aoki and Norihiro Sato日期:01/05/2018,浏览量:3074,Q&A: 0
    [Abstract] Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage of metabolic energy and carbon, and also for the consumption of excess energy (e.g., Hirai ...
    Lipidomic Analysis of Caenorhabditis elegans Embryos
    秀丽隐杆线虫胚胎的脂质组学分析
    作者:Hung-Chi Yang, Cheng-Yu Hung, Yi-Yun Pan, Szecheng J Lo and Daniel Tsun-Yee Chiu日期:09/20/2017,浏览量:4558,Q&A: 0
    [Abstract] Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is ...
    Sterol Analysis in Kluyveromyces lactis
    乳酸克鲁维酵母中的甾醇分析
    作者:Yvetta Gbelska, Nora Toth Hervay, Marcela Morvova and Alexandra Konecna日期:09/05/2017,浏览量:3162,Q&A: 0
    [Abstract] Sterols are essential lipids of most eukaryotic cells with multiple functions (structural, regulatory and developmental). Sterol profile of yeast cells is often determined during the studies of ergosterol synthesis mutants used to uncover a number of functions for various sterols in yeast cells. Molecular studies of ergosterol biosynthesis have ...
    Metabolic Heavy Isotope Labeling to Study Glycerophospholipid Homeostasis of Cultured Cells
    代谢重同位素标记法研究培养细胞的甘油磷脂稳态
    作者:Satu Hänninen, Pentti Somerharju and Martin Hermansson日期:05/05/2017,浏览量:3685,Q&A: 0
    [Abstract] Glycerophospholipids consist of a glycerophosphate backbone to which are esterified two acyl chains and a polar head group. The head group (e.g., choline, ethanolamine, serine or inositol) defines the glycerophospholipid class, while the acyl chains together with the head group define the glycerophospholipid molecular species. Stable ...
    Determination of Rate of [3H-methyl]-choline Incorporation into Cellular Lipids and Non-lipid Metabolites
    [3H-甲基]-胆碱掺入细胞脂质和非脂质代谢物速率的测定
    作者:Tim Andrew Davies Smith and Su Myat Phyu日期:11/20/2016,浏览量:4015,Q&A: 0
    [Abstract] The choline-containing phospholipid, phosphatidylcholine (PtdCho) is the most common mammalian phospholipid found in cell membrane (Ide et al., 2013). It is also a component of intracellular signalling pathways (Cui and Houweling, 2002). Herein is described a measure of the rate of accumulation of choline by lipid soluble PtdCho and ...
    Ionization Properties of Phospholipids Determined by Zeta Potential Measurements
    通过ζ电位测量测定磷脂双分子层的膜电位
    作者:Murugappan Sathappa and Nathan N. Alder日期:11/20/2016,浏览量:7018,Q&A: 0
    [Abstract] Biological membranes are vital for diverse cellular functions such as maintaining cell and organelle structure, selective permeability, active transport, and signaling. The surface charge of the membrane bilayer plays a critical role in these myriad processes. For most biomembranes, the surface charge of anionic phospholipids contributes to the ...
    Fat Turnover Assay in Drosophila
    果蝇的脂肪转化测定
    作者:Subhash D. Katewa and Pankaj Kapahi日期:11/05/2016,浏览量:6484,Q&A: 0
    [Abstract] Like all animals, Drosophila shows robust fat (triglyceride) turnover, i.e., they synthesize, store and utilize triglyceride for their daily metabolic needs. The protocol describes a simple assay to measure this turnover of triglycerides in Drosophila.