植物科学

分类

    现刊
    Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method
    苯酚-硫酸法测定藻类和蓝藻中淀粉/糖原的储存和总糖含量
    作者:Tomáš Zavřel, Petra Očenášová, Maria A. Sinetova and Jan Červený日期:08/05/2018,浏览量:5003,Q&A: 0
    [Abstract] This is a protocol for quantitative determination of storage and total carbohydrates in algae and cyanobacteria. The protocol is simple, fast and sensitive and it requires only few standard chemicals. Great advantage of this protocol is that both storage and total saccharides can be determined in the cellular pellets that were already used for ...
    ROS Detection in Botryococcus braunii Colonies with CellROX Green Reagent
    使用CellROX绿色试剂检测布朗葡萄藻集落中的ROS
    作者:Edmundo Lozoya-Gloria, Ivette Cornejo-Corona, Hem R. Thapa, Daniel R. Browne and Timothy P. Devarenne日期:08/20/2017,浏览量:5313,Q&A: 0
    [Abstract] We analyzed the reactive oxygen species (ROS) accumulation in the colony-forming green microalga Botryococcus braunii in response to several stress inducers such as NaCl, NaHCO3, salicylic acid (SA), methyl jasmonate, and acetic acid. A staining assay using the fluorescent dye CellROX Green was used. CellROX Green is a ...
    Aniline Blue and Calcofluor White Staining of Callose and Cellulose in the Streptophyte Green Algae Zygnema and Klebsormidium
    采用苯胺蓝和Calcofluor White对绿藻类双星藻属和克里藻属的胼胝质和纤维素进行染色
    作者:Klaus Herburger and Andreas Holzinger日期:10/20/2016,浏览量:9053,Q&A: 0
    [Abstract] Plant including green algal cells are surrounded by a cell wall, which is a diverse composite of complex polysaccharides and crucial for their function and survival. Here we describe two simple protocols to visualize callose (1→3-β-D-glucose) and cellulose (1→4-β-D-glucose) and related polysaccharides in the cell walls of streptophyte green algae. ...
    Cryo-focused Ion Beam Sample Preparation for Imaging Vitreous Cells by Cryo-electron Tomography
    低温聚焦离子束方法制备样本用于低温电子断层扫描术观察玻璃化细胞
    作者:Miroslava Schaffer, Benjamin D. Engel, Tim Laugks, Julia Mahamid, Jürgen M. Plitzko and Wolfgang Baumeister日期:09/05/2015,浏览量:15748,Q&A: 0
    [Abstract] Cryo-electron tomography (CET) is a well-established technique for imaging cellular and molecular structures at sub-nanometer resolution. As the method is limited to samples that are thinner than 500 nm, suitable sample preparation is required to attain CET data from larger cell volumes. Recently, cryo-focused ion beam (cryo-FIB) milling of ...
    Ciliary and Flagellar Membrane Vesicle (Ectosome) Purification
    纤毛和鞭毛囊泡(外皮层)的纯化
    作者:William Dentler日期:06/20/2014,浏览量:7783,Q&A: 0
    [Abstract] Eukaryotic cilia/flagella are ideal organelles for the analysis of membrane trafficking, membrane assembly, and the functions of a variety of signal transduction molecules. Cilia are peninsular organelles and the membrane lipids, membrane proteins, and microtubular-associated components are selectively transported into cilia through the region ...
    Deflagellation and Regeneration in Chlamydomonas
    衣藻的鞭毛脱落和再生
    作者:William Dentler日期:06/20/2014,浏览量:11625,Q&A: 0
    [Abstract] Eukaryotic cilia/flagella are one of the only cellular structures that can be removed without injuring cells, can be highly purified for biochemical analysis, and, in many cells, can be completely reassembled within 90 minutes. Following amputation, the expression of many flagellar genes is up-regulated, and many are packaged and associated with ...
    Heat Shock Treatment of Chlamydomonas reinhardtii and Chlorella Cells
    热击处理莱茵衣藻和小球藻细胞
    作者:Stephanie Chankova, Zhana Mitrovska and Nadezhda Yurina日期:08/05/2013,浏览量:7338,Q&A: 0
    [Abstract] The protocol is very reliable and simple for inducing heat shock in unicellular green algae cells. The main purpose was to compare cellular response of three Chlorella species, isolated from different habitats: Chlorella vulgaris 8/1- thermophilic, Chlorella kesslery- mesophilic and C. vulgaris- extremophilic. ...
    Immunoelectron Microscopy in Chlamydomonas Cells
    免疫电镜术检测衣藻属细胞
    作者:Maria A. Sinetova and Alexandra G. Markelova日期:03/05/2013,浏览量:9531,Q&A: 0
    [Abstract] The method of immunoelectron microscopy is intended for localization of proteins inside the cells of Chlamydomonas reinhardtii or other microalgae and cyanobacteria. This protocol was used to study localization of carbonic anhydrase Cah3 with antibodies raised in rabbit, though it can be used to localize any other abundant protein. ...