摘要:通过超薄切片,获得细胞水平的结构;同时通过免疫染色,观察目的蛋白在细胞内的定位及变化情况。
关键词: 胶体金, 免疫定位, 细胞
材料与试剂
注: 以下试剂除特别说明外,均购自Sigma-Aldrich公司。
仪器设备
实验步骤
溶液配方
注:本实验方案中所需的溶液除特殊说明外,均用蒸馏水配制。
致谢
本篇实验方案改编自先前发表的文章 Zhang et al. (2004)。
参考文献
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