Plant Science

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    Colocalization Assay with Fluorescent-tagged ATG8 Using a Nicotiana benthamiana-based Transient System
    Authors:  Jinyan Mai, Dandan Shang, Faqiang Li and Na Luo, date: 08/20/2022, view: 1076, Q&A: 0

    Autophagy is an evolutionarily conserved intracellular degradation process. During autophagy, a set of autophagy-related (ATG) proteins orchestrate the formation of double-bound membrane vesicles called autophagosomes to engulf cytoplasmic material

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    Imaging of Lipid Uptake in Arabidopsis Seedlings Utilizing Fluorescent Lipids and Confocal Microscopy
    Authors:  Rosa L. López-Marqués and Thomas G. Pomorski, date: 11/20/2021, view: 2284, Q&A: 0

    Eukaryotic cells use a diverse set of transporters to control the movement of lipids across their plasma membrane, which drastically affects membrane properties. Various tools and techniques to analyze the activity of these transporters have been

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    A Novel Method to Map Small RNAs with High Resolution
    Authors:  Kun Huang, Feray Demirci, Blake C. Meyers and Jeffrey L. Caplan, date: 08/20/2021, view: 2459, Q&A: 0

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular

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    A Pulse–chase EdU Method for Detection of Cell Division Orientation in Arabidopsis and Juncus prismatocarpus Leaf Primordia
    Authors:  Xiaofeng Yin and Hirokazu Tsukaya, date: 01/05/2021, view: 2588, Q&A: 0
    In plants, the morphological diversity of leaves is largely determined by cell division, especially cell division orientation. Whereas cell division itself is easily monitored, the detection and quantification of cell division orientation are ...
    Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides
    Authors:  Kalina T. Haas, Methieu Rivière, Raymond Wightman and Alexis Peaucelle, date: 10/05/2020, view: 4368, Q&A: 0
    The plant cell wall (PCW) is a pecto-cellulosic extracellular matrix that envelopes the plant cell. By integrating extra-and intra-cellular cues, PCW mediates a plethora of essential physiological functions. Notably, it permits controlled and ...
    Preparing Immunolocalization Slides of Maize Meiotic Chromosomes for Three-dimensional Microscopy
    Authors:  Qian Du and Wojciech P. Pawlowski, date: 07/20/2020, view: 1772, Q&A: 0
    The protocol provides fully detailed steps for preparing microscopic slides of acrylamide-embedded maize meiotic cells. This method is particularly useful for examining chromatin structure and chromosome arrangement without destroying the ...
    Live Cell Imaging of Male Meiosis in Arabidopsis by a Landmark-based System
    Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as cell division. Here, we present a step-by-step protocol for a robust and easy-to-use live cell imaging approach to study male meiosis in the ...
    Chiasma Counting in Maize Male Meiocytes
    Authors:  Gaganpreet K. Sidhu and Wojciech P. Pawlowski, date: 05/05/2020, view: 2556, Q&A: 0
    This protocol combines a classical chromosome spreading technique with 3-dimensional image reconstruction for visualization of bivalent configurations during meiosis to aid chiasma counting.
    Immunolabeling of Maize Meiocytes
    Authors:  Mateusz Zelkowski and Wojciech P. Pawlowski, date: 04/05/2020, view: 3028, Q&A: 0
    This protocol describes a quick method for immunolocalization of meiotic proteins in maize. It includes a fixation step that allows for long-term storage of material and provides good preservation of chromatin structure.
    Tandem Tag Assay Optimized for Semi-automated in vivo Autophagic Activity Measurement in Arabidopsis thaliana roots
    Authors:  Adrian N. Dauphinee, Jonas A. Ohlsson and Elena A. Minina, date: 03/05/2020, view: 3933, Q&A: 0
    Autophagy is the main catabolic process in eukaryotes and plays a key role in cell homeostasis. In vivo measurement of autophagic activity (flux) is a powerful tool for investigating the role of the pathway in organism development and ...



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