摘要:通过获得的目的蛋白抗体,在一定条件下进行抗原抗体结合反应,进行目的蛋白的亚细胞定位实验。本实验一抗为RAC1多克隆抗体,二抗为连接荧光素抗体。通过激光共聚焦扫描显微镜观察,进行RAC1蛋白在青杄花粉管发育过程中细胞定位。
关键词: 蛋白质, 免疫定位, 细胞
材料与试剂
注: 以下试剂除特别说明外,均购自Sigma-Aldrich公司。
仪器设备
实验步骤
注意事项
参考文献
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