Plant Science


    Fluorescence in situ Localization of Pri-miRNAs in Isolated Arabidopsis thaliana Nuclei

    Here, we present an approach combining fluorescence in situ hybridization (FISH) and immunolabeling for localization of pri-miRNAs in isolated nuclei of A. thaliana. The presented method utilizes specific DNA oligonucleotide probes, modified by

    A Protocol for Mitotic Metaphase Chromosome Count Using Shoot Meristematic Tissues of Mulberry Tree Species

    Studies on chromosomal status are a fundamental aspect of plant cytogenetics and breeding because changes in number, size, and shape of chromosomes determine plant physiology/performance. Despite its significance, the classical cytogenetic study is

    Quantification of Botrytis cinerea Growth in Arabidopsis thaliana
    Authors:  Patricia Scholz, Kent D. Chapman, Till Ischebeck and Athanas Guzha, date: 08/20/2023, view: 536, Q&A: 0

    Yield losses attributed to plant pathogens pose a serious threat to plant productivity and food security. Botrytis cinerea is one of the most devastating plant pathogens, infecting a wide array of plant species; it has also been established as a

    Likelihood-based Modeling of Brassiceae Ancient Whole-genome Triplication with POInT
    Authors:  Yue Hao and Gavin C. Conant, date: 08/20/2023, view: 33, Q&A: 0

    Ancient whole-genome duplication and triplication events have a profound impact on present-day plant genomes. However, sub genome assignments in allopolyploids can be technically challenging. The present study used a likelihood-based tool, POInT

    Likelihood Estimation of Arabidopsis Population Parameters with MAPGD
    Authors:  Yue Hao, Matthew S. Ackerman and Michael Lynch, date: 08/20/2023, view: 37, Q&A: 0

    MAPGD ( is a likelihood-based software for population genomics data analysis. Here, we demonstrate a simple tutorial for using MAPGD to analyze Arabidopsis population data. We estimated the allele and genotype

    Simple Growth Complementation Assay in Yeast
    Authors:  Robert Fuhrmeister and Jana Streubel, date: 08/05/2023, view: 208, Q&A: 0

    The study of genes and their products is an essential prerequisite for fundamental research. Characterization can be achieved by analyzing mutants or overexpression lines or by studying the localization and substrate specificities of the resulting

    Inoculation of Maize with Sugarcane Mosaic Virus Constructs and Application for RNA Interference in Fall Armyworms
    Authors:  Iram Gull and Georg Jander, date: 07/20/2023, view: 320, Q&A: 0

    Virus-mediated transient gene overexpression and gene expression silencing can be used to screen gene functions in plants. Sugarcane mosaic virus (SCMV) is a positive strand RNA virus in the Potyviridae family that has been modified to be used as

    Autolysin Production from Chlamydomonas reinhardtii
    Author:  Justin Findinier, date: 07/05/2023, view: 259, Q&A: 1

    Chlamydomonas reinhardtii is a model organism for various processes, from photosynthesis to cilia biogenesis, and a great chassis to learn more about biofuel production. This is due to the width of molecular tools available, which have recently

    Isolation and Quantification of Mandelonitrile from Arabidopsis thaliana Using Gas Chromatography/Mass Spectrometry
    Authors:  Ana Arnaiz, J. Lucas Vallejo-García, Saul Vallejos and Isabel Diaz, date: 06/20/2023, view: 299, Q&A: 0

    Mandelonitrile is a nitrogen-containing compound, considered an essential secondary metabolite. Chemically, it is a cyanohydrin derivative of benzaldehyde, with relevant functions in different physiological processes including defense against

    Metagenomic Protocol (From Quality Control to Mapping) for Metagenome-assembled Genomes Using Anvi’o
    Authors:  Soumyadev Sarkar, Tanner Richie and Sonny T. M. Lee, date: 05/20/2023, view: 78, Q&A: 0

    Synapses provide the main route of signal transduction within neuronal networks. Many factors regulate critical synaptic functions. These include presynaptic calcium channels, triggering neurotransmitter release, and postsynaptic ionotropic


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