摘要:免疫共沉淀是以抗体和抗原之间的专一性作用为基础的用于研究蛋白质相互作用的方法。当细胞在非变性条件下被裂解时,完整细胞内存在的许多蛋白质与蛋白质间的相互作用也被保留下来。用预先固化在beads上的蛋白质A的抗体免疫沉淀A蛋白,那么与A蛋白在体内结合的蛋白质B也能一起沉淀下来,再通过蛋白变性分离,对B蛋白进行检测,进而证明两者间的相互作用。
关键词: 草莓果实, Co-IP, 蛋白互作
材料与试剂
仪器设备
实验步骤
溶液配方
参考文献
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