摘要:酵母双杂交系统可进行两个蛋白的互作分析。真核生物的位点特异性转录激活因子通常含有两个不同的结构域:DNA结合结构域 (DNA-binding domain) 和转录激活结构域 (transcription-activating domain)。前者可识别DNA上的特异序列,并使转录激活结构域定位于所调节基因的上游,转录激活结构域可同转录复合体的其他成分作用, 启动它所调节基因的转录。两个结构域可在适当部位打开且具有各自的功能,当两结构域通过适当的途径在空间上接近时其激活转录的能力可被恢复。将两待检测的蛋白分别与BD和AD融合并导入酵母菌株中,通过营养缺陷筛选可验证两个蛋白之间是否存在相互作用。
关键词: 蛋白互作, 酵母双杂交
材料与试剂
仪器设备
实验步骤
溶液配方
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