实验原理:用农杆菌浸染拟南芥花序,利用授粉受精的过程,将农杆菌导入胚珠,从而结出含有目的基因的转基因种子。农杆菌浸染花序遗传转化方法避免了愈伤组织培养过程,而且可节省大量的时间。 实验目的:通过农杆菌蘸花侵染拟南芥< /b>获得转基因拟南芥植株用于基因功能研究。
关键词: 拟南芥, 农杆菌, 蘸花, 转基因
材料与试剂
仪器设备
实验步骤
注意事项
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