实验原理:植物叶片经过暗处理之后,可以通过PAM荧光仪激发出红色的荧光,再反馈到荧光仪,从而可以获得在PSII不同开放程度下的Fo、Fm等基本荧光参数。 实验目的:通过PAM荧光仪测量高等植物光系统II的最大光化学量子产量Fv/Fm,以揭示植株是否处于正常健康的生理状态。
关键词: 叶绿素荧光参数, Fv/Fm, 胁迫
材料与试剂
仪器设备
实验步骤
本实验方案视频见视频1。
结果与分析
高等植物在没有受到胁迫、正常的生理状态下,Fv/Fm的值在0.80-0.84之间。但是当叶片受到胁迫之后该值会显著下降。
注意事项
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