实验原理:成熟花粉粒含有淀粉粒,遇碘变蓝。 实验目的:鉴定水稻花粉的育性。
关键词: 水稻, 碘-碘化钾, 染色法, 花粉, 育性
材料与试剂
仪器设备
实验步骤
结果与分析
显微镜下观测结果: 可育花粉粒呈圆形、大而饱满、深蓝色、着色均匀;不育花粉包括无花粉型、典败、圆败和染败共四种类型。无花粉型指显微镜下观测,无花粉或仅留残余花粉壁;典败指显微镜下观测花粉不染色,形状不规则状,如三管形、多边形等;圆败指花粉粒外观圆形,无染色淀粉粒;染败指大多数花粉形态正常,但着色较浅或着色不均匀,也有部分花粉深染色,但粒形明显异于正常可育花粉粒。
溶液配方
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