实验原理:免疫染色类似于动物免疫细胞化学,是指带显色剂标记的特异性抗体在组织细胞原位通过抗原抗体反应,对相应抗原进行定性、定位、定量测定的技术。水稻免疫染色基本过程为:将组织酶解分离细胞核后,用特异性抗体免疫细胞核,通过荧光标记的二抗孵育,在荧光显微镜下观测荧光强弱及分布,从而确定抗原蛋白的性质。 实验目的:根据使用抗体的类型,免疫染色可用来检测分析特定细胞时期或不同材料中组蛋白修饰情况,进而测定相关蛋白活性;或特定蛋白细胞核内的精细分布。
关键词: 核提取, 抗体, 激光共聚焦
材料与试剂
仪器设备
实验步骤
溶液配方
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