实验原理:Southern印记杂交 (Southern Blotting) 是将植物基因组总DNA酶切,通过琼脂糖凝胶电泳分离,变性之后利用虹吸原理印记到固相支持物上 (如尼龙膜),然后与变性的同位素标记探针特异性地在一定温度下退火,最后根据同位素标记情况分析杂交结果的分子生物学技术。 实验目的:Southern Blotting可用于多种实验目的,比如检测目的片段拷贝数,限制性片断长度多态性分析、外源基因的整合模式等等,该操作说明适用于水稻的目的片段拷贝数检测。
关键词: Southern blotting, RFLP, 拷贝数检测
材料与试剂
仪器设备
实验步骤
溶液配方
参考文献
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