实验原理:植物体内的DNA通常在细胞核中以核蛋白的形式存在,利用阳离子去垢剂CTAB (hexadecyltrimethylammonium bromide,十六烷基三甲基溴化铵) 与剧烈的高低温差破坏细胞膜结构使这些核蛋白释放出来。利用氯仿等有机试剂去除蛋白、多糖、酚类等杂质,且DNA在高盐 (1 M NaCl溶液) 条件下可溶,最后使用高浓度酒精溶液重新沉淀获得高质量的植物基因组DNA。 实验目的:大量提取植物基因组DNA,可用于Southern blot分析,对转基因植株进行外源基因插入拷贝数和整合模式检测等。
关键词: 总DNA, 大样抽提, Southern blot
材料与试剂
仪器设备
实验步骤
溶液配方
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