Protocols in Current Issue
    Agrobacterium-mediated Transformation of Japonica Rice Using Mature Embryos and Regenerated Transgenic Plants
    Authors:  Ammar Elakhdar, Masako Fukuda and Takahiko Kubo, date: 09/20/2021, view: 1655, Q&A: 0

    Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice. However, the efficiency of gene transfer by Agrobacterium in rice is low, particularly among japonica

    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    [Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both ...
    Gene Expression and Genome Editing Systems by Direct Delivery of Macromolecules Into Rice Egg Cells and Zygotes
    Authors:  Erika Toda and Takashi Okamoto, date: 07/20/2020, view: 2320, Q&A: 0
    [Abstract] Polyethylene glycol calcium (PEG-Ca2+)-mediated transfection allows rapid and efficient examination to analyze diverse cellular functions of genes of interest. In plant cells, macromolecules, such as DNA, RNA and protein, are delivered into protoplasts derived from somatic tissues or calli via PEG-Ca2+ transfection. To ...
    Thin Sections of Technovit 7100 Resin of Rice Endosperm and Staining
    Author:  Ryo Matsushima, date: 09/20/2014, view: 13014, Q&A: 0
    [Abstract] Starch is a biologically and commercially important carbohydrate that is accumulated in plant storage organs, such as seed endosperm. Starch is water-insoluble and forms transparent grains, referred to as starch grains (SGs). SGs are easily stained by iodine solution and can be observed under a normal microscope. Technovit 7100 resin is suitable ...
    Targeted Gene Mutation in Rice Using a CRISPR-Cas9 System
    Authors:  Kabin Xie, Bastian Minkenberg and Yinong Yang, date: 09/05/2014, view: 31113, Q&A: 0
    [Abstract] RNA-guided genome editing (RGE) using bacterial type II cluster regularly interspaced short palindromic repeats (CRISPR)–associated nuclease (Cas) has emerged as a simple and versatile tool for genome editing in many organisms including plant and crop species. In RGE based on the Streptococcus pyogenes CRISPR-Cas9 system, the Cas9 ...
    Isolation of Rice Embryo Single Cell Type using Laser Capture Microdissection (LCM)
    Author:  Tie Liu, date: 09/20/2012, view: 11645, Q&A: 0
    [Abstract] A lot of transcriptional profiling in plant and animals has used RNAs samples from many different cell types. The laser-capture microdissection (LCM) can identify and harvest pure cellular populations directly from heterogenous tissues based on histological identification. The molecules or protein isolated from LCM-captured cells can be suitable ...

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