Ralph Thomas Boettcher

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Akira Karasawa New York Structural Biology Center
6 protocols

Alexandros Kokotos Weill Cornell Medicine
13 protocols

Alka Mehra Institute of Genomics and Integrative Biology
36 protocols

Aruna Bitra La Jolla institute for allergy and immunology
2 protocols

Editor

Ralph Thomas Boettcher

Project group leader, Max Planck Institute for Biochemistry

Research focus

Cell biology

1 Author merit
Reviewer merit
Editor merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=b%C3%B6ttcher+rt

1 Protocol published

β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)

Author: Ralph T. Böttcher, date: 11/05/2013, view: 8827, Q&A: 0

Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...

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5 Protocols reviewed

Correction: Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging

Author: Andrew D. Doyle, date: 05/05/2020, view: 317, Q&A: 0

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Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites

Authors: Christina Wefers, Ghaith Bakdash, Meritxell Moreno Martin, Tjitske Duiveman-de Boer, Ruurd Torensma, Leon F.A.G. Massuger and I. Jolanda M. de Vries, date: 04/05/2017, view: 7552, Q&A: 0

Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...

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42 Protocols edited

Isolation and ex vivo Expansion of Human Limbal Epithelial Progenitor Cells

Authors: Naresh Polisetti, Günther Schlunck, Thomas Reinhard, Friedrich E. Kruse and Ursula Schlötzer-Schrehardt, date: 09/20/2020, view: 333, Q&A: 0

Limbal stem cell transplantation has been used successfully to treat patients with limbal stem cell deficiency all over the world. However, long term clinical results often proved less satisfactory due to the low quality of the graft or inadequate ...

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Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the trans Golgi Network

Authors: Xiao Tang, Feng Yang and Yusong Guo, date: 03/05/2020, view: 1300, Q&A: 0

Protein sorting at the trans Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim of this proposal is to reconstitute the packaging of non-Golgi resident cargo proteins ...

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