摘要:厌氧微生物生活在无氧的环境中,与需氧微生物相比培养技术特殊。本文介绍了厌氧微生物培养基制备方法,具体营养成分需要根据不同微生物的种类进行选择。
关键词: 厌氧微生物, 培养基, 培养
材料与试剂
仪器设备
实验步骤
一、厌氧微生物培养基配制
二、厌氧微生物培养
以瘤胃微生物培养为例 (Hailemariam et al., 2020)
致谢
感谢中国农业科学院创新工程 (ASTIP-IAS12) 支持。采用本实验方法发表的文章如下:Hailemariam, S., Zhao, S. and Wang, J. (2020). Complete genome sequencing and transcriptome analysis of nitrogen metabolism of Succinivibrio dextrinosolvens strain z6 isolated from dairy cow rumen. Front Microbiol 11: 1826.
参考文献
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which is better to use carbon dioxide or nitrogen gas?
Hello, which is better to use carbon dioxide or nitrogen gas? Will carbon dioxide exposure affect the pH adjustment of the culture medium?