摘要:本方法概述了河湖着生硅藻的样品采集、永久玻片制作、显微鉴定及其注意事项,为规范实验操作过程/提高后续相关数据分析的可比性提供依据。本实验以固定面积法采集河湖自然底质表面的着生硅藻样品,通过强酸溶解有机质的方法,去除有机杂物及硅藻细胞内含物,只留下硅藻细胞壁,并利用树胶制作成永久玻片,在显微镜下鉴定、计数,得到硅藻种类及密度数据,用于下游群落分析。
关键词: 着生硅藻, 样品采集, 永久玻片, 鉴定, 计数
材料与试剂
仪器设备
实验步骤
一、样品采集
注意事项
失败经验
溶液配方
参考文献
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.